Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4269096..4269593 Replicon chromosome
Accession NZ_CP073936
Organism Escherichia coli strain MB56

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag KFU61_RS20735 Protein ID WP_001173343.1
Coordinates 4269096..4269269 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag KFU61_RS20740 Protein ID WP_000398619.1
Coordinates 4269297..4269593 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU61_RS20725 (4265766) 4265766..4267223 + 1458 WP_000856835.1 dipeptide/tripeptide permease DtpC -
KFU61_RS20730 (4267460) 4267460..4268977 + 1518 WP_001295087.1 lysine--tRNA ligase -
KFU61_RS20735 (4269096) 4269096..4269269 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
KFU61_RS20740 (4269297) 4269297..4269593 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
KFU61_RS20745 (4269820) 4269820..4270092 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
KFU61_RS20750 (4270104) 4270104..4270334 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
KFU61_RS20755 (4270515) 4270515..4272146 + 1632 WP_001216477.1 sensor histidine kinase -
KFU61_RS20760 (4272143) 4272143..4272862 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T200666 WP_001173343.1 NZ_CP073936:c4269269-4269096 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T200666 NZ_CP098136:c2641630-2641346 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCAGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT200666 WP_000398619.1 NZ_CP073936:c4269593-4269297 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT200666 NZ_CP098136:c2641862-2641620 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCAGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References