Detailed information of TA system TA200662

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2426895..2427533
• Replicon: chromosome
• Accession: NZ_CP073936
• Organism: Escherichia coli strain MB56

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: KFU61_RS11665
• Protein ID: WP_000813794.1
• Coordinates: 2427357..2427533 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: KFU61_RS11660
• Protein ID: WP_001270286.1
• Coordinates: 2426895..2427311 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU61_RS11640 (2422047)	2422047..2422988	-	942	WP_001251304.1	ABC transporter permease	-
KFU61_RS11645 (2422989)	2422989..2424002	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
KFU61_RS11650 (2424020)	2424020..2425165	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
KFU61_RS11655 (2425410)	2425410..2426816	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
KFU61_RS11660 (2426895)	2426895..2427311	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
KFU61_RS11665 (2427357)	2427357..2427533	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
KFU61_RS11670 (2427755)	2427755..2427985	+	231	WP_000494244.1	YncJ family protein	-
KFU61_RS11675 (2428077)	2428077..2430038	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
KFU61_RS11680 (2430111)	2430111..2430647	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
KFU61_RS11685 (2430700)	2430700..2431914	+	1215	WP_001326689.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T200662 WP_000813794.1 NZ_CP073936:c2427533-2427357 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T200662 NZ_CP098135:197281-197565 [Klebsiella pneumoniae]
ATGACTTATAAGCTGGCCTTTAATGAATCAGCTCTGAAAGAATGGAAAAAATTGGGCCATACCATTCGGGAACAATTCAA
AAAGAAGCTCGCTGAGCGCCTGTTGAACCCACGGGTGCCCGCATCGCAACTACATGGACGCAAAGACCAATACAAAATAA
AGCTCCGTGGCGCAGGCTACCGACTGGTTTACAGTGTCAACGATGATGTTGTGACGGTCACCGTAATTGGCGTCGGTAAA
CGTGAGAATGATGATATTTACAATTTAACCAAACACAGAAATTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT200662 WP_001270286.1 NZ_CP073936:c2427311-2426895 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT200662 NZ_CP098135:197043-197291 [Klebsiella pneumoniae]
ATGGCATTTCAGGTACTAACCACTACTGCAGCAAGCATCACAGAGCTTAAACGTGATCCTATGGGCACTTTCAATGCAGG
AGAAGGTGCCCCTGTAGCCATACTGAATCGCAACGAACCCGCTTTTTACTGTGTACCGCCTGCTCTGTATGCTCAACTGA
TGGAGCTTCTTGAAGATCAGGAGCTGGGTCGTATTGCTGATGAGCGAATTGGTGAGGCGGTAATCGAGGTCAGTCTAGAT
GACTTATAA

Structures

Toxin

• PDB: 6HPB