Detailed information of TA system TA200661

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2340100..2341688
• Replicon: chromosome
• Accession: NZ_CP073936
• Organism: Escherichia coli strain MB56

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: S1ELM3
• Locus tag: KFU61_RS11290
• Protein ID: WP_001125439.1
• Coordinates: 2340366..2341688 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: KFU61_RS11285
• Protein ID: WP_001301023.1
• Coordinates: 2340100..2340366 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU61_RS11285 (2340100)	2340100..2340366	+	267	WP_001301023.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
KFU61_RS11290 (2340366)	2340366..2341688	+	1323	WP_001125439.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
KFU61_RS11295 (2342000)	2342000..2342208	+	209	Protein_2206	AraC family transcriptional regulator	-
KFU61_RS11300 (2342463)	2342463..2343293	+	831	Protein_2207	fimbria/pilus outer membrane usher protein	-
KFU61_RS11305 (2343274)	2343274..2343471	+	198	Protein_2208	type 1 fimbrial protein	-
KFU61_RS11310 (2343484)	2343484..2343987	+	504	WP_000825452.1	fimbrial protein	-
KFU61_RS11315 (2344046)	2344046..2344960	+	915	WP_000520676.1	fimbrial protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-401)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49275.82 Da
• Isoelectric Point: 8.2482

>T200661 WP_001125439.1 NZ_CP073936:2340366-2341688 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTHPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVESNVLRLHGRLSREYGSK

Nucleotide

• Length: 1323 bp

>T200661 NZ_CP098135:c136541-136059 [Klebsiella pneumoniae]
ATGGGAATGAGAGCGCCTGAATCCCTGACGGCTGAACATAACATCGCTGAGTTCTGCTGCCAGGAGCCTGCCCTTAATGA
GTGGTTAAAGAAGAAGGCTCTCAGAAACCACAGTACCGGCATTTCCCGTGTCTATGTCGTCTGCGCAGAAAACACTAACC
GGGTCATCGGTTATTATTGTTTATCTTCGGGTAGCGTCCACCGTAACACAGTGCCTGGGGCCTATAGACGCAACGCGCCT
GATGTCATTCCAGTCATTGTTTTGGGACGGCTTGCCATCGACCAGGCCTGGGCCGGGAATGGGCTTGGTGCCGCACTTCT
TAAGGATGCTATATACCGTACGCAAAACATAGCCTTTCAGGTCGGGGTGAGAGCACTGGCCGTGCATGCGCTGAATGAAG
AAGTTAAATGTTTTTACACCCGTTTTGGCTTTGAGCCTTCCATAGTCAACACGCTGACATTGTTATTCCCGATAAAAGTC
TGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10016.38 Da
• Isoelectric Point: 6.2267

>AT200661 WP_001301023.1 NZ_CP073936:2340100-2340366 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDAKNASPES
TEQQNLEW

Nucleotide

• Length: 267 bp

>AT200661 NZ_CP098135:c136810-136532 [Klebsiella pneumoniae]
ATGAAACCAGACGTTCGTGAAGCTCCGATTAATATCCGGGCCAAAACATCACAGCGTGACCTGATCGACATGGCCGCAAG
CCTGGTCTCAAAATCACGGACTGATTTTATGCTTGAGGTTGCCTGCAGGGAGGCACAGGACATTCTGCTTGATCAACGCC
TGTTCCTCCTCAATGACAATCAGTTTGAGGCGTTTATTGAAGAGCTTGACGCCCCAATAACGCCGGAGCGCCAGGCGCGA
ATCGATAACCTGATGAACCGCAAATCACCATGGGAATGA

Structures

Toxin

• PDB: 5K98
• PDB: 3TPV
• PDB: 3DNV
• PDB: 3TPD
• PDB: 3HZI
• PDB: 3FBR
• PDB: 4YG7
• PDB: 3TPE
• PDB: 2WIU
• PDB: 3DNT
• PDB: 3TPB
• PDB: 3TPT
• PDB: 3DNU