Detailed information of TA system TA020065

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 1397815..1398453
• Replicon: chromosome
• Accession: HG738867
• Organism: Escherichia coli str. K-12 substr. MC4100

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: BN896_1262
• Protein ID: CDJ71843.1
• Coordinates: 1397815..1397991 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: BN896_1263
• Protein ID: CDJ71844.1
• Coordinates: 1398037..1398453 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BN896_1258	1393434..1394609	-	1176	CDJ71839.1	putative benzoate transporter	-
BN896_1259	1394701..1395237	+	537	CDJ71840.1	putative DNA-binding transcriptional regulator	-
BN896_1260	1395310..1397271	+	1962	CDJ71841.1	putative peptidase	-
BN896_1261	1397363..1397593	-	231	CDJ71842.1	hypothetical protein	-
BN896_1262	1397815..1397991	+	177	CDJ71843.1	hypothetical protein	Toxin
BN896_1263	1398037..1398453	+	417	CDJ71844.1	putative DNA-binding transcriptional regulator	Antitoxin
BN896_1264	1398532..1399938	+	1407	CDJ71845.1	fused putative DNA-binding transcriptional regulator/putative amino transferase	-
BN896_1265	1400183..1401328	+	1146	CDJ71846.1	putative spermidine/putrescine transporter subunit	-
BN896_1266	1401346..1402359	+	1014	CDJ71847.1	putative spermidine/putrescine transporter subunit	-
BN896_1267	1402360..1403301	+	942	CDJ71848.1	putative spermidine/putrescine transporter subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T20065 CDJ71843.1 HG738867:1397815-1397991 [Escherichia coli str. K-12 substr. MC4100]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T20065 HG738867:1397815-1397991 [Escherichia coli str. K-12 substr. MC4100]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT20065 CDJ71844.1 HG738867:1398037-1398453 [Escherichia coli str. K-12 substr. MC4100]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT20065 HG738867:1398037-1398453 [Escherichia coli str. K-12 substr. MC4100]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA