Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3904079..3904655 Replicon chromosome
Accession NZ_CP073932
Organism Escherichia coli strain MB57

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag KFU62_RS18815 Protein ID WP_001297643.1
Coordinates 3904368..3904655 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag KFU62_RS18810 Protein ID WP_000063156.1
Coordinates 3904079..3904381 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU62_RS18795 (3900675) 3900675..3902825 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
KFU62_RS18800 (3902875) 3902875..3903078 + 204 WP_000467859.1 YbdD/YjiX family protein -
KFU62_RS18805 (3903089) 3903089..3904045 + 957 WP_001297640.1 GTPase -
KFU62_RS18810 (3904079) 3904079..3904381 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
KFU62_RS18815 (3904368) 3904368..3904655 - 288 WP_001297643.1 BrnT family toxin Toxin
KFU62_RS18820 (3904854) 3904854..3905551 - 698 Protein_3674 IS1 family transposase -
KFU62_RS18825 (3905608) 3905608..3905703 + 96 Protein_3675 dienelactone hydrolase family protein -
KFU62_RS18830 (3905849) 3905849..3907081 + 1233 WP_001380906.1 multidrug efflux MFS transporter MdtM -
KFU62_RS18835 (3907122) 3907122..3908402 + 1281 WP_001037380.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3904854..3905063 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T200640 WP_001297643.1 NZ_CP073932:c3904655-3904368 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T200640 NZ_CP098128:c3766384-3765968 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATTATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACCGACATCCGGGTGGCGCTGCACCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGCCACGCCATCGCGGCGGGCGCCGTCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTGCCTGGACTGACACTGG
AAGACTGGGTTATTTAG

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT200640 WP_000063156.1 NZ_CP073932:c3904381-3904079 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT200640 NZ_CP098128:c3766611-3766381 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAACGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References