Detailed information of TA system TA200624

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ypjF-yeeU/CbtA-CbeA
• Location: 1107242..1107974
• Replicon: chromosome
• Accession: NZ_CP073932
• Organism: Escherichia coli strain MB57

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A377CSV0
• Locus tag: KFU62_RS05360
• Protein ID: WP_001194227.1
• Coordinates: 1107242..1107571 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: KFU62_RS05365
• Protein ID: WP_001059334.1
• Coordinates: 1107609..1107974 (-)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU62_RS05355 (1102298)	1102298..1106878	+	4581	WP_001581967.1	adhesin-like autotransporter YpjA/EhaD	-
KFU62_RS05360 (1107242)	1107242..1107571	-	330	WP_001194227.1	TA system toxin CbtA family protein	Toxin
KFU62_RS05365 (1107609)	1107609..1107974	-	366	WP_001059334.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
KFU62_RS05370 (1108000)	1108000..1108221	-	222	WP_000691984.1	DUF987 domain-containing protein	-
KFU62_RS05375 (1108218)	1108218..1108760	-	543	WP_001098974.1	DNA repair protein RadC	-
KFU62_RS05380 (1108773)	1108773..1109216	-	444	WP_000381004.1	antirestriction protein	-
KFU62_RS05385 (1109247)	1109247..1110068	-	822	WP_001234410.1	DUF932 domain-containing protein	-
KFU62_RS05390 (1110160)	1110160..1110501	-	342	WP_000720952.1	hypothetical protein	-
KFU62_RS05395 (1110536)	1110536..1111000	-	465	WP_000791790.1	hypothetical protein	-
KFU62_RS05400 (1111126)	1111126..1111284	-	159	WP_001128372.1	hypothetical protein	-
KFU62_RS05405 (1111497)	1111497..1112384	-	888	WP_001567853.1	50S ribosome-binding GTPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	luxS	1053464..1132274	78810

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-108)

Antitoxin

• Antitoxin CbeA (17-117)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12445.11 Da
• Isoelectric Point: 7.2767

>T200624 WP_001194227.1 NZ_CP073932:c1107571-1107242 [Escherichia coli]
MQTLSSHPTRATQPCLSPVETWQRLLTHLLSQHYGLTLNDTPFSNETTIREDIDAGVSLSDAVNFLVEKYELVRIDRKGF
SWQEQTPYISVVDILRARRSTGLLKTNVK

Nucleotide

• Length: 330 bp

>T200624 NZ_CP098123:3988810-3989028 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 13527.37 Da
• Isoelectric Point: 5.8806

>AT200624 WP_001059334.1 NZ_CP073932:c1107974-1107609 [Escherichia coli]
MNNHSVFGTKPENLTCQEWGLKRTITPCFGARLVQEGNRVHFLADRAGFNGAFIDDNALRLDLAFPLILKQLELILTSGE
LSPRHEHCVTLYHNGLTCEADTLGSCGYVYIAIYPDQPEPH

Nucleotide

• Length: 366 bp

>AT200624 NZ_CP098123:3988409-3988783 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: A0A377CSV0