Detailed information of TA system TA200618

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 77851..78452
• Replicon: plasmid pYLMB64a
• Accession: NZ_CP073930
• Organism: Escherichia coli strain MB64

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: KFU91_RS23270
• Protein ID: WP_072731915.1
• Coordinates: 77851..78231 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: KFU91_RS23275
• Protein ID: WP_001190712.1
• Coordinates: 78231..78452 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU91_RS23235 (KFU91_23050)	72926..73486	-	561	WP_001161490.1	recombinase family protein	-
KFU91_RS23240 (KFU91_23055)	73796..75373	+	1578	WP_001323889.1	PAS domain-containing methyl-accepting chemotaxis protein	-
KFU91_RS23245	75589..76017	-	429	Protein_75	ORF6N domain-containing protein	-
KFU91_RS23250 (KFU91_23070)	76119..76319	-	201	WP_089586254.1	host cell division inhibitor Icd-like protein	-
KFU91_RS23255 (KFU91_23075)	76325..76507	-	183	Protein_77	phage antirepressor Ant	-
KFU91_RS23260 (KFU91_23080)	76596..77639	-	1044	WP_211131004.1	DUF968 domain-containing protein	-
KFU91_RS23265 (KFU91_23085)	77667..77846	-	180	WP_000113018.1	hypothetical protein	-
KFU91_RS23270 (KFU91_23090)	77851..78231	-	381	WP_072731915.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
KFU91_RS23275 (KFU91_23095)	78231..78452	-	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
KFU91_RS23280 (KFU91_23100)	78635..80191	+	1557	WP_089586248.1	type I restriction-modification system subunit M	-
KFU91_RS23285 (KFU91_23105)	80188..81327	+	1140	WP_089586244.1	restriction endonuclease subunit S	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	tet(A) / aph(3'')-Ib / aph(6)-Id	-	1..112470	112470

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (2-123)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13631.32 Da
• Isoelectric Point: 5.1514

>T200618 WP_072731915.1 NZ_CP073930:c78231-77851 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSSE

Nucleotide

• Length: 381 bp

>T200618 NZ_CP098123:405386-405733 [Klebsiella pneumoniae]
ATGTGGGATGTGGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCTTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCAATAAGGATGGCATAAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT200618 WP_001190712.1 NZ_CP073930:c78452-78231 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT200618 NZ_CP098123:405733-406032 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGAAAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGACTGATAACGACCCTCGGCTTTCGACCCTAAAAAGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CJB6