Detailed information of TA system TA200617

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 43222..43757
• Replicon: plasmid pYLMB64a
• Accession: NZ_CP073930
• Organism: Escherichia coli strain MB64

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: KFU91_RS23090
• Protein ID: WP_032206513.1
• Coordinates: 43222..43509 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: T1PXV2
• Locus tag: KFU91_RS23095
• Protein ID: WP_001139206.1
• Coordinates: 43506..43757 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU91_RS23075 (KFU91_22890)	38847..42362	+	3516	WP_072752622.1	autotransporter outer membrane beta-barrel domain-containing protein	-
KFU91_RS23080 (KFU91_22895)	42537..42742	-	206	Protein_42	hypothetical protein	-
KFU91_RS23085	42741..42854	+	114	Protein_43	alkaline phosphatase	-
KFU91_RS23090 (KFU91_22905)	43222..43509	-	288	WP_032206513.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KFU91_RS23095 (KFU91_22910)	43506..43757	-	252	WP_001139206.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
KFU91_RS23100 (KFU91_22915)	44339..46921	+	2583	Protein_46	helicase-related protein	-
KFU91_RS23105 (KFU91_22920)	46977..48190	+	1214	WP_257199204.1	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	tet(A) / aph(3'')-Ib / aph(6)-Id	-	1..112470	112470

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11130.20 Da
• Isoelectric Point: 10.5833

>T200617 WP_032206513.1 NZ_CP073930:c43509-43222 [Escherichia coli]
MTYTVKFRDDALKEWLRLDKTIQQQFAKKLKKCSENPHIASAKLRGLKDCYKIKLRASGFRLVYQIIDDMLIIAVVAVGK
RERSNVYHLASERMK

Nucleotide

• Length: 288 bp

>T200617 NZ_CP098123:379417-379785 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGACAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9171.39 Da
• Isoelectric Point: 4.0927

>AT200617 WP_001139206.1 NZ_CP073930:c43757-43506 [Escherichia coli]
MPSIILSDTSASVSELKKNPMATVSAGEGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSSQPLHDVDLD
SYL

Nucleotide

• Length: 252 bp

>AT200617 NZ_CP098123:379199-379420 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Antitoxin

• AlphaFold DB: T1PXV2