Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4291713..4292210 Replicon chromosome
Accession NZ_CP073929
Organism Escherichia coli strain MB64

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag KFU91_RS20610 Protein ID WP_001173343.1
Coordinates 4291713..4291886 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag KFU91_RS20615 Protein ID WP_000398619.1
Coordinates 4291914..4292210 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU91_RS20600 (4288378) 4288378..4289835 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
KFU91_RS20605 (4290072) 4290072..4291589 + 1518 WP_001295074.1 lysine--tRNA ligase -
KFU91_RS20610 (4291713) 4291713..4291886 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
KFU91_RS20615 (4291914) 4291914..4292210 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
KFU91_RS20620 (4292437) 4292437..4292709 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
KFU91_RS20625 (4292721) 4292721..4292951 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
KFU91_RS20630 (4293132) 4293132..4294763 + 1632 WP_104769000.1 sensor histidine kinase -
KFU91_RS20635 (4294760) 4294760..4295479 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T200616 WP_001173343.1 NZ_CP073929:c4291886-4291713 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T200616 NZ_CP098042:32189-32467 [Providencia rettgeri]
ATGATAAAATCTTTCAAGCACAAAGGATTGAAACTGCTTTTTGAAAAGGGTGTTACTTCTGGTGTGCCTGCGCAAGATGT
AGACAGAATCAATGACCGTTTGCAGGCCATCGATACAGCGACAGAGATTGGTGAACTAAACCGCCAGATTTACAAATTAC
ATCCATTAAAGGGGGATCGGGAAGGTTACTGGTCTATCACTGTCCGGGCGAATTGGCGCATTACTTTTCAGTTCATTAAC
GGTGATGCTTACATTTTAAATTATGAGGATTATCACTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT200616 WP_000398619.1 NZ_CP073929:c4292210-4291914 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT200616 NZ_CP098042:32467-32781 [Providencia rettgeri]
ATGAGACAATTCAAGGTTTCACATCCGGGTGAGATGATAGCCCGCGATTTAGAGGATATGGGAGTGAGTGGTCGTCGTTT
TGCTCACAATATTGGTGTTACACCAGCAACCGTATCCCGTCTACTTGCGGGGAAAACTGCGTTGACCCCTTCTCTTTCGA
TTCGTATTGCTGCGGCACTGGGGAGTACGCCTGAGTTTTGGTTACGGCTACAGAGCAATTATGATCTGCGCCAGTTAGAA
AACCAAATCGATACATCCGGGATCGTCTTGTACGGTGAGTCGAACGAACAGCAGCAGAACGCGCAAGAGCATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References