Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4099700..4100276 Replicon chromosome
Accession NZ_CP073929
Organism Escherichia coli strain MB64

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag KFU91_RS19665 Protein ID WP_001297643.1
Coordinates 4099989..4100276 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag KFU91_RS19660 Protein ID WP_000063156.1
Coordinates 4099700..4100002 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU91_RS19645 (4096296) 4096296..4098446 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
KFU91_RS19650 (4098496) 4098496..4098699 + 204 WP_000467859.1 YbdD/YjiX family protein -
KFU91_RS19655 (4098710) 4098710..4099666 + 957 WP_001297640.1 GTPase -
KFU91_RS19660 (4099700) 4099700..4100002 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
KFU91_RS19665 (4099989) 4099989..4100276 - 288 WP_001297643.1 BrnT family toxin Toxin
KFU91_RS19670 (4100475) 4100475..4101172 - 698 Protein_3844 IS1 family transposase -
KFU91_RS19675 (4101229) 4101229..4101324 + 96 Protein_3845 dienelactone hydrolase family protein -
KFU91_RS19680 (4101470) 4101470..4102702 + 1233 WP_024186283.1 multidrug efflux MFS transporter MdtM -
KFU91_RS19685 (4102743) 4102743..4104023 + 1281 WP_001037400.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T200615 WP_001297643.1 NZ_CP073929:c4100276-4099989 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T200615 NZ_CP098041:c102179-101829 [Providencia rettgeri]
ATGTGGGAAATATTAACGAGAGATCTCTTTGATTCTTGGTTCGAAGAGCAAAATGAGGAAACTCAGATTGAAGTGTTGGC
AGTGTTAATGATTTTACGTGAGGATGGCCCCAATCTTGGACGCCCTCAAGTTGACACCCTGAAGGGCTCACAGTTTCCCA
ACATGAAGGAACTGCGAATTCAGGTTGGTGGTCATCCGATTAGAGCGTGTTTCGCATTTGACCCAATTCGTCGTGGCATT
GTGTTATGTGCCGGTGATAAGAAAGGAAAAGATGAAACCCGTTTCTACAAGAAATTAATCAAGATGGCCGATGCGGAATA
TGCAGCGCACTTATTAGAGCAGGAGAAATAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT200615 WP_000063156.1 NZ_CP073929:c4100002-4099700 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT200615 NZ_CP098041:c101827-101522 [Providencia rettgeri]
ATGTCCACTTTAAATGAACTACTTGCAAAACAATCCCCCGAAACGCTGGCAAAAATTGAAGACCGAGCAGAAGAAATCCG
GCGTGAAATCACACTAGCTAAAATTCGTGAGGAACTGAAACTTTCTCAAACTGAGCTAGCTCAATCACTGGGCGTTTCGC
AACCTTCTATTGCTAAGCTTGAAAATGTTGATAACGATCCGAAGCTTTCAACTCTGAAGCGCTACATTAAAGCTTTAGGC
GGTGAACTTTCTATCGATGTAACGTTACCGAATGGTAAGAGAATTGGATTGCATCTTTGAGCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References