Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 95891..96534 Replicon plasmid pYLMB98a
Accession NZ_CP073927
Organism Escherichia coli strain MB98

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag KFU77_RS24075 Protein ID WP_001034044.1
Coordinates 96118..96534 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag KFU77_RS24070 Protein ID WP_001261286.1
Coordinates 95891..96121 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU77_RS24050 (92522) 92522..93277 - 756 WP_000852146.1 replication initiation protein RepE -
KFU77_RS24055 (93999) 93999..94805 - 807 WP_000016970.1 site-specific integrase -
KFU77_RS24060 (94806) 94806..95111 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB -
KFU77_RS24065 (95113) 95113..95331 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA -
KFU77_RS24070 (95891) 95891..96121 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
KFU77_RS24075 (96118) 96118..96534 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
KFU77_RS24080 (96609) 96609..98174 + 1566 WP_001128474.1 AAA family ATPase -
KFU77_RS24085 (98159) 98159..99181 + 1023 WP_000361402.1 helicase UvrD -
KFU77_RS24090 (99435) 99435..100121 - 687 Protein_120 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(B) / mph(A) / blaCTX-M-15 / aac(3)-IIa / blaOXA-1 / aac(6')-Ib-cr / sitABCD iutA / iucD / iucC / iucB / iucA 1..118380 118380
- flank IS/Tn - - 99435..99782 347


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T200592 WP_001034044.1 NZ_CP073927:96118-96534 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T200592 NZ_CP098030:c3478393-3478040 [Serratia ureilytica]
GTGTGGGAAATTAAAACAACGGATGCATTTGATCATTGGTTCAGATCACTCAGCGATGCCGACCGGGCCTGCGTGCTTGC
TGCATTGATGGTCCTGCGGGAAAAAGGGCCCGGCTTGCCCAGACCCTATGCCGATACGGTTAAAGGCTCGCGTTACAGCA
ACATGAAAGAGCTGCGCATTCAGTGCCGTGGCGAACCGTTGCGGGCATTCTTCGCCTTCGATCCGTACCGCGTCGGCATG
GTGCTTTGCGCGGGCAATAAAGCGGGCGATGAAAAACGTTTTTACGATCGAATGCTGCAGATTGCCGATCGGGAATTCAC
GAATTGGCTGAACACACTAAAAGAGAAGGAGTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT200592 WP_001261286.1 NZ_CP073927:95891-96121 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT200592 NZ_CP098030:c3478035-3477733 [Serratia ureilytica]
ATGGGCAGAACGCTGGAACAGCTTATTGCGGAGGAAAAGCCCGAGGTCGTGGCCAAAGCGCAGGCGCTGGCGGCGGACAT
CATGCTGAACATTCACCTTGCCGAGCTGCGCGAGAAGGTGCAGAAAACCCAGGTTGAAATGGCGCAGGCGCTGGGGATCA
GGCAGCCGACCGTTGCCGTGATGGAAAAACCGGGCCGCGATCTGAAACTGTCTTCGCTTAAACGCTATGTTGAGGCGGCC
GGCGGCAAACTGCGCCTGGACATCGAACTGCCGGACGGCTCTCACTACGAGTTTGTGCTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References