Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3545910..3546528 Replicon chromosome
Accession NZ_CP073926
Organism Escherichia coli strain MB98

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag KFU77_RS17175 Protein ID WP_001291435.1
Coordinates 3546310..3546528 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag KFU77_RS17170 Protein ID WP_000344800.1
Coordinates 3545910..3546284 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU77_RS17160 (3540999) 3540999..3542192 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KFU77_RS17165 (3542215) 3542215..3545364 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
KFU77_RS17170 (3545910) 3545910..3546284 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
KFU77_RS17175 (3546310) 3546310..3546528 + 219 WP_001291435.1 HHA domain-containing protein Toxin
KFU77_RS17180 (3546700) 3546700..3547251 + 552 WP_000102568.1 maltose O-acetyltransferase -
KFU77_RS17185 (3547367) 3547367..3547837 + 471 WP_000136192.1 YlaC family protein -
KFU77_RS17190 (3548001) 3548001..3549551 + 1551 WP_001372022.1 cyclic-guanylate-specific phosphodiesterase PdeB -
KFU77_RS17195 (3549593) 3549593..3549946 - 354 WP_000878140.1 DUF1428 domain-containing protein -
KFU77_RS17205 (3550325) 3550325..3550636 + 312 WP_000409911.1 MGMT family protein -
KFU77_RS17210 (3550667) 3550667..3551239 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T200584 WP_001291435.1 NZ_CP073926:3546310-3546528 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T200584 NZ_CP098030:184708-184983 [Serratia ureilytica]
ATGCTCAGCATCGAATGGACTCGAGCCGCACGTCAGGATCTGGCGGAGATTGTGGGATTCATCGCCAATGACAATCCTTT
GGCGGCCAGAAAAATGAAGCTTCTCATCATGGAGGCGGTCATTCCCGCCGCCAGTCATCCTCGACTTTTCCGGCGTGGCA
GAGTGGACGGCACGCATGAAATTCCTCTACATCCCAATTATCTCTTGGTTTATCAAGTGCTGGAAACTCACATCCGGGTG
TTGAGGGTTTTACATGCGCGCCGACAATATCCTTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT200584 WP_000344800.1 NZ_CP073926:3545910-3546284 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT200584 NZ_CP098030:184508-184708 [Serratia ureilytica]
ATGATGAGTATTTTACTACTGCCTACGGTTGCCAAGATGGAAAATGAGGAACACACGCAGGGCTATGAGCGCTGGTTTCT
TGAAAAAGTTCAGGAAGCCATGGATGAGCCATTGCCCGGTATTCCTCATGATGACGTGATGGCTGAAATGGATGCTGTTA
TCGCTGCTGCAGAAGCCAGGATCAAAGCTAAACAGTCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References