Detailed information of TA system TA200572

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1408537..1409162
• Replicon: chromosome
• Accession: NZ_CP073926
• Organism: Escherichia coli strain MB98

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KFU77_RS06860
• Protein ID: WP_000911330.1
• Coordinates: 1408764..1409162 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: KFU77_RS06855
• Protein ID: WP_000450524.1
• Coordinates: 1408537..1408764 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU77_RS06830 (1404340)	1404340..1404810	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
KFU77_RS06835 (1404810)	1404810..1405382	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
KFU77_RS06840 (1405528)	1405528..1406406	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
KFU77_RS06845 (1406423)	1406423..1407457	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
KFU77_RS06850 (1407670)	1407670..1408383	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
KFU77_RS06855 (1408537)	1408537..1408764	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
KFU77_RS06860 (1408764)	1408764..1409162	+	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
KFU77_RS06865 (1409309)	1409309..1410172	+	864	WP_001267507.1	neutral zinc metallopeptidase	-
KFU77_RS06870 (1410187)	1410187..1412202	+	2016	WP_000829323.1	tRNA cytosine(34) acetyltransferase TmcA	-
KFU77_RS06875 (1412276)	1412276..1412974	+	699	WP_000679823.1	esterase	-
KFU77_RS06880 (1413084)	1413084..1413284	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T200572 WP_000911330.1 NZ_CP073926:1408764-1409162 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T200572 NZ_CP098025:1912751-1913095 [Enterococcus faecalis]
ATGAAAAGTATCAAAGAGTTGGTGGAAGAATATGAAGTAGAGTTAGTTTTCGCTCCAATAAATAAGCGCGCATGTTACGA
GCCAGTCAAAAGAATAATTTTCGTAAATCAAAATTTATCTATTGAAGAACAAGAAGAGTCTATATTCCACGAGTTCAAGC
ATGTTGTTTCCCATTCGGATTACATGGAATTATATAAAATTCCTTCTTTTAGAAATAAAATGGAAGCTGAGGCAGATCAT
CATATGTTTAAATGCCTTATTGAAAAACATGACGGGCAATTTAATTACTCTAATGTGATTACTCATTACAATTTAAAGAT
GGGACAGGAAACTTATTTAAAATAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT200572 WP_000450524.1 NZ_CP073926:1408537-1408764 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT200572 NZ_CP098025:1912401-1912733 [Enterococcus faecalis]
ATGAACCTTTTGGAACGTATAAAAAAACTAGCAAAAGATAGAGATGTGTCTATTTATCAGCTTGAAGAAGAAATAAATAT
TGGACGAAATACTATTTATCAATGGAATAAGCGAACACCTTCTTCGGATAAACTTGAAGCTGTAGCCAATTATTTTAATG
TTTCTGTTGATTATTTATTAGGTCGTACTGAAAATCCAAACCAAGCTGGTGCTAAACCATCTGATGATTTAGATGAAGTA
TTAGACAATGTGATGAGTTTCGATGGTGAGCCTTTGGATGATCATGATCGGGAAGTTATTCGTGCTTATTTGAAAGGGAG
ATTCGGAAAATAA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33