Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 323384..323970 Replicon chromosome
Accession NZ_CP073926
Organism Escherichia coli strain MB98

Toxin (Protein)


Gene name hicA Uniprot ID A0A0H0RQZ4
Locus tag KFU77_RS01485 Protein ID WP_042014162.1
Coordinates 323384..323614 (+) Length 77 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag KFU77_RS01490 Protein ID WP_000593555.1
Coordinates 323611..323970 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU77_RS01475 (319421) 319421..322156 + 2736 WP_000149132.1 ribosome-associated ATPase/putative transporter RbbA -
KFU77_RS01480 (322156) 322156..323280 + 1125 WP_001314210.1 ABC transporter permease -
KFU77_RS01485 (323384) 323384..323614 + 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin Toxin
KFU77_RS01490 (323611) 323611..323970 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
KFU77_RS01495 (324090) 324090..324491 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
KFU77_RS01500 (324497) 324497..325303 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
KFU77_RS01505 (325300) 325300..326064 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
KFU77_RS01510 (326064) 326064..326897 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
KFU77_RS01515 (326894) 326894..327838 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(15-65)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 77 a.a.        Molecular weight: 8419.79 Da        Isoelectric Point: 8.5518

>T200564 WP_042014162.1 NZ_CP073926:323384-323614 [Escherichia coli]
MDQLFKSPLPQGIKWTDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP

Download         Length: 231 bp

>T200564 NZ_CP097983:c1248336-1248118 [Pantoea alhagi]
ATGACAGAAAAAATTATGACCAAGACTGACTATCTGATGCGCTTAAGACGTTGCCGATCCATTGATACACTTGAGCGTGT
TATTGAAAAGAATAAATATGAATTACCTGATGATGAACTGGCTGTTTTTTATTCCGCAGCGGATCATCGCCTGGCTGAAT
TAACTATGAATAAGCTGTACGATAAAGTCCCAGTATCAGTATGGAAATATGTACGGTAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT200564 WP_000593555.1 NZ_CP073926:323611-323970 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT200564 NZ_CP097983:c1248744-1248364 [Pantoea alhagi]
ATGGACGAATACTCACCGAAACGGCATGATATTGCCCAGTTGAATTTCTTGTGTGAGAATCTGTACGACGAAAGTATGGC
AACGTTAGGTGACAGCCATCATGGCTGGGTTAATGATCCTACGTCCGCTTCTAATTTACAACTGAATGATCTGATTGAAC
ATATTGCTTCATTCACGATGAATTATAAAATTAAGCATGCTGAGGATGAGGATCTCATTGTTCAAATTGATGATTACCTC
GATGATACCTTTATGCTTTTCAGTAATTACGGCATCAATGTTCAGGACCTACAACGCTGGCAGCGTTCCGCCAGACGTTT
GTTTAACCTTTTCGCAGAAGAGTGCGCTCAGCTTCCTTTACAGCCAAGCCATTCATTTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H0RQZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References