Detailed information of TA system TA200558

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3535203..3536040
• Replicon: chromosome
• Accession: NZ_CP073924
• Organism: Escherichia coli strain MB165

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: KFU71_RS17045
• Protein ID: WP_000227784.1
• Coordinates: 3535498..3536040 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: KFU71_RS17040
• Protein ID: WP_001297137.1
• Coordinates: 3535203..3535514 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU71_RS17015 (3530223)	3530223..3531170	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
KFU71_RS17020 (3531192)	3531192..3533183	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
KFU71_RS17025 (3533173)	3533173..3533787	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
KFU71_RS17030 (3533787)	3533787..3534116	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
KFU71_RS17035 (3534128)	3534128..3535018	+	891	WP_000971332.1	heme o synthase	-
KFU71_RS17040 (3535203)	3535203..3535514	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
KFU71_RS17045 (3535498)	3535498..3536040	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
KFU71_RS17050 (3536096)	3536096..3537031	-	936	WP_001297127.1	tetratricopeptide repeat protein	-
KFU71_RS17055 (3537439)	3537439..3538803	+	1365	WP_001000978.1	MFS transporter	-
KFU71_RS17060 (3538931)	3538931..3539422	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
KFU71_RS17065 (3539590)	3539590..3540501	+	912	WP_000705874.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T200558 WP_000227784.1 NZ_CP073924:3535498-3536040 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T200558 NZ_CP097897:c85843-85481 [Acetobacterium wieringae]
TTGCACAAAATACTTTTTTATAAAGACCCAAAAGGCAATGAGCCAGTATTGGATTTCATCAGAGAATTAAGCAAAAAAAA
CGATAAGGACAGCCGTGTTAATCTCAATAAGATCAATGATTACATCCAAATGCTCAGTAAATACGGTACAAAGATCGGTG
AGCCATACATCAAACATTTAGAGAGCGAAATCTGGGAGATCAGGCCCTTAAGAAATAGAATCTTCTTCGTCGCCTGGGTA
GATGGTAGTTATGTATTGCTACACCAATTTATAAAGAAATCACAAAAGACCCCTAAACGAGAAATCGAACAAGCCAAACG
AGAATTGGCATTCTTAAAAGAAAGTGGTGAAAATAATGAATAA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT200558 WP_001297137.1 NZ_CP073924:3535203-3535514 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT200558 NZ_CP097897:c85488-85129 [Acetobacterium wieringae]
ATGAATAATAAAGCAATTGGTAGTAGCTGGACCGATGTCAGAAAAGAAATTTTTACACCTGAAGAAATTCTGGAATCAGA
TTTAAGGGTTGCTCTCATGGGTGAAATCATTAAAGCACGCCATGATAAAGGGATTAGCCAGAAAAAGCTTGAAGAATTAA
GTGGTGTCAGACAGCCAGTGATTGCCAGAATGGAAAGAGGTTCTACCGATCCCAAATTAACAACAGTCCTGAAAATTTTG
GAACCGTTAGGAATGACTTTGACGATTGTGCCCATAAAGGAAAAACGCATGCGTCTTAAACCGCCGTTTGAACTCAATCT
TTCTAAAAATGGCGGTAAACATCCTATAAAAAGAGGCTAG

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3