Detailed information of TA system TA200556

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2538334..2538972
• Replicon: chromosome
• Accession: NZ_CP073924
• Organism: Escherichia coli strain MB165

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: KFU71_RS12305
• Protein ID: WP_000813794.1
• Coordinates: 2538796..2538972 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: KFU71_RS12300
• Protein ID: WP_001270285.1
• Coordinates: 2538334..2538750 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU71_RS12280 (2533486)	2533486..2534427	-	942	WP_001251313.1	ABC transporter permease	-
KFU71_RS12285 (2534428)	2534428..2535441	-	1014	WP_000220411.1	ABC transporter ATP-binding protein	-
KFU71_RS12290 (2535459)	2535459..2536604	-	1146	WP_000034363.1	ABC transporter substrate-binding protein	-
KFU71_RS12295 (2536849)	2536849..2538255	-	1407	WP_000760590.1	PLP-dependent aminotransferase family protein	-
KFU71_RS12300 (2538334)	2538334..2538750	-	417	WP_001270285.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
KFU71_RS12305 (2538796)	2538796..2538972	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
KFU71_RS12310 (2539194)	2539194..2539424	+	231	WP_000494244.1	YncJ family protein	-
KFU71_RS12315 (2539516)	2539516..2541477	-	1962	WP_001307191.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
KFU71_RS12320 (2541550)	2541550..2542086	-	537	WP_000429153.1	DNA-binding transcriptional regulator SutR	-
KFU71_RS12325 (2542178)	2542178..2543353	+	1176	WP_001236265.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2543393..2544541	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (88-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T200556 WP_000813794.1 NZ_CP073924:c2538972-2538796 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T200556 NZ_CP097896:2399271-2399741 [Pectobacterium actinidiae]
TTGATCCTCTATCGGCTGACAAAGACAAAGTATCTTTCTACAGCCTGGACGGGATACGGAGCAAAAGAGGCGGGAGGTCG
CTGGAACAGTGTCGGCACCTCGATGGTTTATGTCTCAGAAACCGCATCGCTGACTATGCTGGAGACGCTGGTACACCTGC
ACGCAGCGCAGATCCTGGATTATTACACGCTGATGCGTATCGATGTGCCTGAAGATCAGATTCAGAGTGCCAATATGGAC
GAATTGCCAGACAACTGGGCTGCTGAGGAAGCACCACCGGATCTGGCGGTGTACGGTGATGCGTGGATCTTTACCCAAAG
TTCTATCGCGTTGCGAGTCCCCAGCGCCCTCTCGCCCGTAGAATATAACTATTTGTTAAACCCTGAACACCCGGATTTCT
ACGGGATTATTCAGAGAGTAGAGGTTATACCGTTCCAGTTTGATCGTCGGCTAAAGCCCGAACGTAAATAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15247.61 Da
• Isoelectric Point: 4.6115

>AT200556 WP_001270285.1 NZ_CP073924:c2538750-2538334 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFDLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT200556 NZ_CP097896:2398831-2399274 [Pectobacterium actinidiae]
ATGAAAACATTTAGCTTATCGACGACGCAGACTCGGCCGCCCCGTTTATGGCAGGTGGCCGGATTACACCATTCCGATGG
TGTAGCGCTTCTGGGACAGATTCGCGAAGGCCTTGAGGGCAATGTTGCTAACCTTATCGCTGACTGGGCGAAGATAACGC
AAAGCGACCTGCGTAAAATGTCCGGTATTCCGTCAACGACATTTAGCCGCAGCGTCAAAGCAAGATTTAACGCCGATCAA
AGCGAACGGCTAGTGCGTATCATCCGTGTTATCGATCGGGCGGTAGAGTTGTTCGAAGGTGATAAAGACGACGCTCAAAA
ATGGTTGAATGAACCCAACAGGGCGCTTGGCGGAAAAATACCAACAGAACTGATGGTATCCGAAACCGGGGCTTATGAAG
TCATGAAATTGCTCACGCGTCTGGAGCATGGTGTGTACTCTTGA

Structures

Toxin

• PDB: 6HPB