Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 935280..935934 Replicon chromosome
Accession NZ_CP073924
Organism Escherichia coli strain MB165

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag KFU71_RS04575 Protein ID WP_000244772.1
Coordinates 935527..935934 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag KFU71_RS04570 Protein ID WP_000354046.1
Coordinates 935280..935546 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU71_RS04545 (930449) 930449..931192 + 744 WP_000951941.1 SDR family oxidoreductase -
KFU71_RS04550 (931249) 931249..932682 - 1434 WP_001307385.1 6-phospho-beta-glucosidase BglA -
KFU71_RS04555 (932727) 932727..933038 + 312 WP_001182945.1 N(4)-acetylcytidine aminohydrolase -
KFU71_RS04560 (933202) 933202..933861 + 660 WP_000250274.1 hemolysin III family protein -
KFU71_RS04565 (934057) 934057..935037 - 981 WP_000886095.1 tRNA-modifying protein YgfZ -
KFU71_RS04570 (935280) 935280..935546 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
KFU71_RS04575 (935527) 935527..935934 + 408 WP_000244772.1 protein YgfX Toxin
KFU71_RS04580 (935974) 935974..936495 - 522 WP_001055874.1 flavodoxin FldB -
KFU71_RS04585 (936607) 936607..937503 + 897 WP_000806637.1 site-specific tyrosine recombinase XerD -
KFU71_RS04590 (937528) 937528..938238 + 711 WP_000715208.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
KFU71_RS04595 (938244) 938244..939977 + 1734 WP_000813220.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T200543 WP_000244772.1 NZ_CP073924:935527-935934 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T200543 NZ_CP097896:c591573-591286 [Pectobacterium actinidiae]
ATGAGTTATCGCGTCAAATTCAGGGAAGATGCGCTCAAAGAATGGCTCAAGCTCGATAAAACCATTCAGCAGCAATTCGC
CAAAAAACTAAAAAAATGCTGCGAAAATCCCCATATTCCTTCGGCAAAATTACGGGGGATGAAGGATTGCTACAAAATCA
CGCTCCGGGCGTCAGGCTTTCGTTTGGTTTACGAAATCATTGACGACATATTGGTTATCGCCGTGGTGGCGGTAGGCAAA
CGTGAGCGCAGTGGCGTTTATCATCTGGCAAGTGAGCGGATGAGATAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT200543 WP_000354046.1 NZ_CP073924:935280-935546 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT200543 NZ_CP097896:c591821-591570 [Pectobacterium actinidiae]
ATGCCGAATATTATATTGAGTGATACCAGTGCCAGCATCAGTGAACTGAAAAAGAACCCAATGGCGACGGTCAATGCTGG
TGATGGCCTGCCTGTTGCTATTTTGAACCGCAACCAGCCTGCGTTTTATTGCATTCCTGCGGCTCTCTACGAAAAAATGC
TGGATGCGCTGGACGATCAGGAATTGGTTAAGCTGATCGGCGAACGTAAAAACCAGCCTCTGGTTGACGTGGATTTGGAT
AGTTTTCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References