Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 291983..292614 Replicon chromosome
Accession NZ_CP073924
Organism Escherichia coli strain MB165

Toxin (Protein)


Gene name hicA Uniprot ID F4NNK6
Locus tag KFU71_RS01315 Protein ID WP_001259388.1
Coordinates 291983..292258 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag KFU71_RS01320 Protein ID WP_000593555.1
Coordinates 292255..292614 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU71_RS01300 (286987) 286987..288054 + 1068 WP_000361482.1 HlyD family efflux transporter periplasmic adaptor subunit -
KFU71_RS01305 (288051) 288051..290786 + 2736 WP_000149132.1 ribosome-associated ATPase/putative transporter RbbA -
KFU71_RS01310 (290786) 290786..291910 + 1125 WP_001615305.1 ABC transporter permease -
KFU71_RS01315 (291983) 291983..292258 + 276 WP_001259388.1 type II toxin-antitoxin system HicA family toxin Toxin
KFU71_RS01320 (292255) 292255..292614 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
KFU71_RS01325 (292734) 292734..293135 - 402 WP_001190064.1 nickel-responsive transcriptional regulator NikR -
KFU71_RS01330 (293141) 293141..293947 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
KFU71_RS01335 (293944) 293944..294708 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
KFU71_RS01340 (294708) 294708..295541 - 834 WP_112014838.1 nickel ABC transporter permease subunit NikC -
KFU71_RS01345 (295538) 295538..296482 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 274282..292614 18332


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10151.89 Da        Isoelectric Point: 10.7076

>T200540 WP_001259388.1 NZ_CP073924:291983-292258 [Escherichia coli]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T200540 NZ_CP097887:914776-915132 [Staphylococcus arlettae]
ATGAGACGAGGAGACGTCTACTTAGCTGACTTATCACCAGTACAAGGGTCTGAACAAGGGGGAGTAAGACCAGTTGTAAT
TATTCAAAATGATACTGGTAATAAATATAGTCCTACCGTGATTGTAGCTGCAATCACTGGTAGGATAAATAAAGCTAAAA
TACCAACACATGTTGAAATTGAAAAGAAAAAGTATAAATTGGATAAGGATTCAGTTATATTACTTGAGCAGATTCGAACG
TTAGATAAAAAAAGATTAAAAGAAAAGTTAACATACTTATCAGAAGATAAAATGAAAGAAGTCAACATAGCAATTGACAT
AAGTTTAGGATTACATAATAATAGGCATCATAAGTAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT200540 WP_000593555.1 NZ_CP073924:292255-292614 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT200540 NZ_CP097887:914606-914776 [Staphylococcus arlettae]
ATGCCAAGTTTTAACCAAAGTAGAAATCATAACATAGAACAATCTTTAAAAGAAGGCTATTCCCAAATGGCTGATTTGAA
TCTCTCCCTTGCAGCAGAAGCTTTTCCATTCGAATGTGAAGCTTGTGATTGCAACGAATCATATATTAGTGCTAGTAACA
CTAATGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FFA2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References