Detailed information of TA system TA020054

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/-
• Location: 262551..263230
• Replicon: chromosome
• Accession: HG738867
• Organism: Escherichia coli str. K-12 substr. MC4100

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: BN896_0226
• Protein ID: CDJ70814.1
• Coordinates: 262551..262892 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: -
• Locus tag: BN896_0227
• Protein ID: CDJ70815.1
• Coordinates: 262913..263230 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BN896_0222	257828..258229	+	402	CDJ70810.1	DNA-binding transcriptional regulator Crl	-
BN896_0223	258268..259323	-	1056	CDJ70811.1	outer membrane phosphoporin protein E	-
BN896_0224	259611..260714	+	1104	CDJ70812.1	gamma-glutamyl kinase	-
BN896_0225	260726..261979	+	1254	CDJ70813.1	gamma-glutamyl phosphate reductase	-
BN896_0226	262551..262892	-	342	CDJ70814.1	CP4-6 prophage; toxin of the YkfI-YafW toxin-antitoxin system	Toxin
BN896_0227	262913..263230	-	318	CDJ70815.1	CP4-6 prophage; antitoxin of the YkfI-YafW toxin-antitoxin system	Antitoxin
BN896_0228	263249..263470	-	222	CDJ70816.1	hypothetical protein	-
BN896_0229	263479..263955	-	477	CDJ70817.1	CP4-6 prophage; putative DNA repair protein	-
BN896_0230	263971..264429	-	459	CDJ70818.1	CP4-6 prophage protein	-
BN896_0231	264527..264766	-	240	CDJ70819.1	CP4-6 prophage protein	-
BN896_0232	264843..265310	-	468	CDJ70820.1	CP4-6 prophage protein	-
BN896_0233	265333..265776	-	444	CDJ70821.1	CP4-6 prophage; putative DNA-binding transcriptional regulator	-
BN896_0234	266407..267228	-	822	CDJ70822.1	CP4-6 prophage protein	-
BN896_0235	267320..268183	-	864	CDJ70823.1	CP4-6 prophage; putative GTP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12905.01 Da
• Isoelectric Point: 9.6543

>T20054 CDJ70814.1 HG738867:c262892-262551 [Escherichia coli str. K-12 substr. MC4100]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T20054 HG738867:c262892-262551 [Escherichia coli str. K-12 substr. MC4100]
ATGAAAACTTTACCTGCAATAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11939.59 Da
• Isoelectric Point: 6.7391

>AT20054 CDJ70815.1 HG738867:c263230-262913 [Escherichia coli str. K-12 substr. MC4100]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT20054 HG738867:c263230-262913 [Escherichia coli str. K-12 substr. MC4100]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCTCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA