Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 1708439..1709090 Replicon chromosome
Accession NZ_CP073916
Organism Lacticaseibacillus paracasei subsp. tolerans strain 2A

Toxin (Protein)


Gene name pemK Uniprot ID K6S8L5
Locus tag KFU60_RS08275 Protein ID WP_003567661.1
Coordinates 1708439..1708822 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID K6RKI5
Locus tag KFU60_RS08280 Protein ID WP_003567665.1
Coordinates 1708842..1709090 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU60_RS08270 (KFU60_08270) 1707734..1708255 - 522 WP_108298683.1 QueT transporter family protein -
KFU60_RS08275 (KFU60_08275) 1708439..1708822 - 384 WP_003567661.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
KFU60_RS08280 (KFU60_08280) 1708842..1709090 - 249 WP_003567665.1 antitoxin Antitoxin
KFU60_RS08285 (KFU60_08285) 1709158..1710294 - 1137 WP_003596242.1 alanine racemase -
KFU60_RS08290 (KFU60_08290) 1710281..1710655 - 375 WP_003567669.1 holo-ACP synthase -
KFU60_RS08295 (KFU60_08295) 1710825..1712333 - 1509 WP_003567670.1 DEAD/DEAH box helicase -
KFU60_RS08300 (KFU60_08300) 1712459..1712602 - 144 WP_003567673.1 hypothetical protein -
KFU60_RS08305 (KFU60_08305) 1712633..1714021 - 1389 WP_108298682.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 13773.03 Da        Isoelectric Point: 10.9764

>T200539 WP_003567661.1 NZ_CP073916:c1708822-1708439 [Lacticaseibacillus paracasei subsp. tolerans]
MDVSVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSTKMAAVDRALAISVGLVSLPKPKTYNKN

Download         Length: 384 bp

>T200539 NZ_CP097887:903794-904000 [Staphylococcus arlettae]
ATGGAATCAAAAGTCATCTTTTTAGCGGGTTTAACGTCTTATAAACAACAAGCACAATTAACACAAAAATTGTTAAATAT
TATTGGAGTGCATCAAGTACACGTTAGCCTCGAGAAAAGTATTGTTCAGATTACATTTGATACACCAGCTAACTTAAATA
ATATCGAAAAAGAAATATATGATGATGGATATACCATTCTATTTTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8938.08 Da        Isoelectric Point: 4.4610

>AT200539 WP_003567665.1 NZ_CP073916:c1709090-1708842 [Lacticaseibacillus paracasei subsp. tolerans]
MKKQTSVAVTFDDAIKARLDAYCELNDIDAQAVITEAVATFLKSQAPEINQLISGYVAMGQINTEISHAFSACESEAYAH
IR

Download         Length: 249 bp

>AT200539 NZ_CP097887:903489-903782 [Staphylococcus arlettae]
ATGACAGAGGTAGACAAAGCGCATCATTCTACTCAGACAAAAGCCAATTTAAAATCACGTTTGAATCGTATAGAGGGGCA
AGTAAGAGCGATTAATAGAATGATTGATGAAGATGTTTATTGTGATGATGTCTTAACACAAATCAGAGCAACGCGTTCTG
CGTTAAACAGTGTTGCTACAAAATTATTAGATCATCATATGAAGAGCTGTATTATGGACAAAGTGAACGCGGGCGAGCAA
GAAGCGGCTATGGAAGAATTACTAGTAACATTTCAAAAGCTCATGAAAGATTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K6S8L5

Antitoxin

Source ID Structure
AlphaFold DB A0A0E2BNY2

References