Detailed information of TA system TA200537

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/MazF(toxin)
• Location: 2243810..2244461
• Replicon: chromosome
• Accession: NZ_CP073915
• Organism: Lacticaseibacillus rhamnosus strain AS

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: K8Q4Y0
• Locus tag: KFU59_RS10430
• Protein ID: WP_005686631.1
• Coordinates: 2243810..2244193 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: K8QH19
• Locus tag: KFU59_RS10435
• Protein ID: WP_005686632.1
• Coordinates: 2244213..2244461 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU59_RS10425 (KFU59_10425)	2242846..2243373	-	528	WP_005711058.1	QueT transporter family protein	-
KFU59_RS10430 (KFU59_10430)	2243810..2244193	-	384	WP_005686631.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KFU59_RS10435 (KFU59_10435)	2244213..2244461	-	249	WP_005686632.1	antitoxin	Antitoxin
KFU59_RS10440 (KFU59_10440)	2244574..2245713	-	1140	WP_005691215.1	alanine racemase	-
KFU59_RS10445 (KFU59_10445)	2245700..2246074	-	375	WP_005686634.1	holo-ACP synthase	-
KFU59_RS10450 (KFU59_10450)	2246243..2247751	-	1509	WP_005686635.1	DEAD/DEAH box helicase	-
KFU59_RS10455 (KFU59_10455)	2248025..2249413	-	1389	WP_014571607.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-115)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 13757.03 Da
• Isoelectric Point: 10.9764

>T200537 WP_005686631.1 NZ_CP073915:c2244193-2243810 [Lacticaseibacillus rhamnosus]
MDVTVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSAKMAAVDRALAISVGLVSLPKPKTYNKN

Nucleotide

• Length: 384 bp

>T200537 NZ_CP097885:c871717-871469 [Peptoniphilus sp. SAHP1]
ATGACTTACCAAGTAAGAACCACAAAAAAGTTTGACAAGTCTTTTAAAAAACTTGATAGGCAGTCACAAAAACTTATAAA
AAATTGGATGGATGGCAATCTTATTGGTTGTCAAAACGCAAGACTTTATGGCAAGGCCCTCACTGGCAACTTCAAAGGGG
TTTGGAGGTATAGGATTGGTGCTTATAGGCTCCTAGCCAAAATTGACGACGACAAGTTAATAATCTTTTTTATAGACAGA
AGAAATTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8936.13 Da
• Isoelectric Point: 4.4778

>AT200537 WP_005686632.1 NZ_CP073915:c2244461-2244213 [Lacticaseibacillus rhamnosus]
MKKETSVTVALDSAIKARLDAYCELNDMGVQDVITEAVAKFLQSQAPEINQLISGYVAMGQINTEISHAFSACESEAYAH
IR

Nucleotide

• Length: 249 bp

>AT200537 NZ_CP097885:c871872-871714 [Peptoniphilus sp. SAHP1]
ATGGGCGAACCACTCTCTACTCTTTTTAAGAGATTGATGGAAGAGAAGCTTGAAGATGAGTTCGACATGAAGGTGGCTGA
GGACTTTCTTGAAAGAGAAGCTAGGGGCGAAGTTGAAACTAGACCTATTGAAGATTTATTTAAAGAGCTTGACCTATGA

Structures

Toxin

• AlphaFold DB: K8Q4Y0

Antitoxin

• AlphaFold DB: K8QH19