Detailed information of TA system TA200530

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/GNAT-DUF1778
• Location: 2322098..2322843
• Replicon: chromosome
• Accession: NZ_CP073914
• Organism: Proteus terrae subsp. cibarius strain SH19PTE4

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: KF949_RS10935
• Protein ID: WP_075673025.1
• Coordinates: 2322352..2322843 (+)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: KF949_RS10930
• Protein ID: WP_075673024.1
• Coordinates: 2322098..2322364 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KF949_RS10895 (KF949_10895)	2317759..2318241	+	483	WP_006533151.1	flagellar basal body-associated protein FliL	-
KF949_RS10900 (KF949_10900)	2318247..2319278	+	1032	WP_023581831.1	flagellar motor switch protein FliM	-
KF949_RS10905 (KF949_10905)	2319271..2319681	+	411	WP_006533153.1	flagellar motor switch protein FliN	-
KF949_RS10910 (KF949_10910)	2319685..2320131	+	447	WP_075673022.1	flagellar biosynthetic protein FliO	-
KF949_RS10915 (KF949_10915)	2320131..2320901	+	771	WP_023581828.1	flagellar type III secretion system pore protein FliP	-
KF949_RS10920 (KF949_10920)	2320917..2321186	+	270	WP_069367481.1	flagellar biosynthesis protein FliQ	-
KF949_RS10925 (KF949_10925)	2321192..2321974	+	783	WP_075673023.1	flagellar biosynthetic protein FliR	-
KF949_RS10930 (KF949_10930)	2322098..2322364	+	267	WP_075673024.1	DUF1778 domain-containing protein	Antitoxin
KF949_RS10935 (KF949_10935)	2322352..2322843	+	492	WP_075673025.1	GNAT family N-acetyltransferase	Toxin
KF949_RS10940 (KF949_10940)	2322929..2323873	-	945	WP_087802138.1	flagellar hook-associated protein FlgL	-
KF949_RS10945 (KF949_10945)	2323898..2325541	-	1644	WP_075673027.1	flagellar hook-associated protein FlgK	-
KF949_RS10950 (KF949_10950)	2325660..2326646	-	987	WP_211838410.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
KF949_RS10955 (KF949_10955)	2326646..2327752	-	1107	WP_075673029.1	flagellar basal body P-ring protein FlgI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (22-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-87)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 18228.15 Da
• Isoelectric Point: 10.3194

>T200530 WP_075673025.1 NZ_CP073914:2322352-2322843 [Proteus terrae subsp. cibarius]
MGKVTAPEPLSNSHEVADFYSSENVLDNWIKQRGLKNQFLGASRTFVVCEKNKKRVVGYYSLATGSVNHTEAMSHIRRNM
PDPIPVIILARLAVDKTFHGKGLGADLLRDAVLRCHHVAENIGVRAIMVHALTENAKHFYLHHGFKASSTQDKILFLALK
KKI

Nucleotide

• Length: 492 bp

>T200530 NZ_CP097884:c2984127-2983951 [Escherichia coli str. K-12 substr. MG1655]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10111.62 Da
• Isoelectric Point: 4.5256

>AT200530 WP_075673024.1 NZ_CP073914:2322098-2322364 [Proteus terrae subsp. cibarius]
MKADVQLNIRAKESQRALIDTAAEILHKSRTDFILETACQAAEDVILDRRTFNLNDAQYAEFIEIIDAPVDIDPALEKLL
MRKPLWEK

Nucleotide

• Length: 267 bp

>AT200530 NZ_CP097884:c2983905-2983489 [Escherichia coli str. K-12 substr. MG1655]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA