200529

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4745876..4746467 Replicon chromosome
Accession NZ_CP073910
Organism Sphingobium phenoxybenzoativorans strain Tas13

Toxin (Protein)


Gene name shpA Uniprot ID -
Locus tag KFK14_RS23505 Protein ID WP_212609356.1
Coordinates 4746171..4746467 (-) Length 99 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID -
Locus tag KFK14_RS23500 Protein ID WP_212609355.1
Coordinates 4745876..4746190 (-) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFK14_RS23475 (KFK14_23475) 4741124..4743073 + 1950 WP_212609350.1 ribonucleoside-diphosphate reductase subunit alpha -
KFK14_RS23480 (KFK14_23480) 4743121..4743525 + 405 WP_212609351.1 endonuclease domain-containing protein -
KFK14_RS23485 (KFK14_23485) 4743603..4743854 - 252 WP_212609352.1 DUF2171 domain-containing protein -
KFK14_RS23490 (KFK14_23490) 4743955..4745007 + 1053 WP_212609353.1 ribonucleotide-diphosphate reductase subunit beta -
KFK14_RS23495 (KFK14_23495) 4745011..4745871 + 861 WP_212609354.1 hypothetical protein -
KFK14_RS23500 (KFK14_23500) 4745876..4746190 - 315 WP_212609355.1 BrnA antitoxin family protein Antitoxin
KFK14_RS23505 (KFK14_23505) 4746171..4746467 - 297 WP_212609356.1 BrnT family toxin Toxin
KFK14_RS23510 (KFK14_23510) 4746530..4746835 - 306 WP_212609357.1 hypothetical protein -
KFK14_RS23515 (KFK14_23515) 4747038..4747259 + 222 WP_212611289.1 hypothetical protein -
KFK14_RS23520 (KFK14_23520) 4747367..4747756 + 390 WP_212609358.1 PH domain-containing protein -
KFK14_RS23525 (KFK14_23525) 4747760..4748095 - 336 WP_212609359.1 DMT family protein -
KFK14_RS23530 (KFK14_23530) 4748235..4748957 + 723 WP_246524765.1 hypothetical protein -
KFK14_RS23535 (KFK14_23535) 4749380..4751164 + 1785 WP_212609360.1 AMP-dependent synthetase/ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-89)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(42-97)


Sequences

Toxin        


Download         Length: 99 a.a.        Molecular weight: 11253.73 Da        Isoelectric Point: 7.2064

>T200529 WP_212609356.1 NZ_CP073910:c4746467-4746171 [Sphingobium phenoxybenzoativorans]
MDMRFEWDPDKAASNRAKHGVSFDLAMRAFADPFALSVQDRIEGGEFRWQLLGMVEGRVLLLVAHTVRDERDGGETIRII
SARRATAGERRRYEQQAF

Download         Length: 297 bp

>T200529 NZ_CP097884:2901237-2902559 [Escherichia coli str. K-12 substr. MG1655]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCAAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCCAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATACCCGAAGA
CGAAACCGTAACGCATCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGCTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACAGCACTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAATGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATGCTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAATTCCAGGTCTTCCAGTGGTTGATTGGCGCAACGGACGGTCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTTTGGCGACAGCAAAGGTGCTGAGATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCA
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAAGCAATGTGTT
GAGGTTGCATGGACGGTTAAGCCGAGAATACGGTAGTAAGTGA

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 11893.49 Da        Isoelectric Point: 5.9137

>AT200529 WP_212609355.1 NZ_CP073910:c4746190-4745876 [Sphingobium phenoxybenzoativorans]
MNNRLSEKRMDFAAPPPLDAELEAELVALEAMDDARIDTSDIAEQGDAFWRDAERGRFYRPLKQSTTVRIDADVLHWLKA
KGKGYQTRINAILREAMMRDGGKR

Download         Length: 315 bp

>AT200529 NZ_CP097884:2900971-2901237 [Escherichia coli str. K-12 substr. MG1655]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTGGCGAATGCAATGAAACTGGTTCGCCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATTGGTATTAAGCAGGCGACGATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACGCGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAAATCTGGAGTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References