Detailed information of TA system TA200527

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parE_1-relB/RelE(toxin)
• Location: 4073191..4073642
• Replicon: chromosome
• Accession: NZ_CP073910
• Organism: Sphingobium phenoxybenzoativorans strain Tas13

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: -
• Locus tag: KFK14_RS20240
• Protein ID: WP_139139438.1
• Coordinates: 4073367..4073642 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: KFK14_RS20235
• Protein ID: WP_212608963.1
• Coordinates: 4073191..4073367 (+)
• Length: 59 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFK14_RS20225 (KFK14_20225)	4069139..4072141	+	3003	WP_212608961.1	excinuclease ABC subunit UvrA	-
KFK14_RS20230 (KFK14_20230)	4072150..4073034	-	885	WP_212608962.1	hypothetical protein	-
KFK14_RS20235 (KFK14_20235)	4073191..4073367	+	177	WP_212608963.1	stability determinant	Antitoxin
KFK14_RS20240 (KFK14_20240)	4073367..4073642	+	276	WP_139139438.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KFK14_RS20245 (KFK14_20245)	4073726..4075261	+	1536	WP_212608964.1	hypothetical protein	-
KFK14_RS20250 (KFK14_20250)	4075486..4075686	+	201	WP_212608965.1	hypothetical protein	-
KFK14_RS20255 (KFK14_20255)	4075860..4076276	+	417	WP_212608966.1	glycine zipper 2TM domain-containing protein	-
KFK14_RS20260 (KFK14_20260)	4076751..4076915	+	165	WP_171905887.1	hypothetical protein	-
KFK14_RS20265 (KFK14_20265)	4076920..4077321	-	402	WP_212608967.1	low affinity iron permease family protein	-
KFK14_RS20270 (KFK14_20270)	4077402..4077734	+	333	WP_070156987.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-90)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10283.81 Da
• Isoelectric Point: 6.9871

>T200527 WP_139139438.1 NZ_CP073910:4073367-4073642 [Sphingobium phenoxybenzoativorans]
MLALIWHPEALDDLENILDFIEVQNPSAAGRIGEAIRHTAGQLPNHPYMYRTGRVPGTREALVTPNYILVYRVAAHAIEI
LAVKHTRQQYP

Nucleotide

• Length: 276 bp

>T200527 NZ_CP097884:2570241-2570344 [Escherichia coli str. K-12 substr. MG1655]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 59 a.a.
• Molecular weight: 6644.53 Da
• Isoelectric Point: 4.7023

>AT200527 WP_212608963.1 NZ_CP073910:4073191-4073367 [Sphingobium phenoxybenzoativorans]
MTKLTSIESEFATTEEAEAYDAWFRATVEARMASDKPGIPHDEVMARMQAIIDRRKQL

Nucleotide

• Length: 177 bp

>AT200527 NZ_CP097884:c2570382-2570111 [Escherichia coli str. K-12 substr. MG1655]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT