Detailed information of TA system TA200526

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 3930822..3931477
• Replicon: chromosome
• Accession: NZ_CP073910
• Organism: Sphingobium phenoxybenzoativorans strain Tas13

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KFK14_RS19420
• Protein ID: WP_212608836.1
• Coordinates: 3930822..3931256 (-)
• Length: 145 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: KFK14_RS19425
• Protein ID: WP_212608837.1
• Coordinates: 3931253..3931477 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFK14_RS19390 (KFK14_19390)	3926813..3927328	-	516	WP_212608831.1	DUF2285 domain-containing protein	-
KFK14_RS19395 (KFK14_19395)	3927390..3927599	-	210	WP_212608832.1	DUF6499 domain-containing protein	-
KFK14_RS19400 (KFK14_19400)	3927858..3928634	+	777	WP_212608833.1	LuxR family transcriptional regulator	-
KFK14_RS19405 (KFK14_19405)	3928691..3929311	+	621	WP_212608834.1	acyl-homoserine-lactone synthase	-
KFK14_RS19410 (KFK14_19410)	3929325..3930200	+	876	WP_212611239.1	phytanoyl-CoA dioxygenase family protein	-
KFK14_RS19415 (KFK14_19415)	3930197..3930808	+	612	WP_212608835.1	GntR family transcriptional regulator	-
KFK14_RS19420 (KFK14_19420)	3930822..3931256	-	435	WP_212608836.1	PIN domain-containing protein	Toxin
KFK14_RS19425 (KFK14_19425)	3931253..3931477	-	225	WP_212608837.1	hypothetical protein	Antitoxin
KFK14_RS24275 (KFK14_19430)	3931700..3931828	+	129	WP_212608838.1	benenodin family lasso peptide	-
KFK14_RS19435 (KFK14_19435)	3931897..3932553	+	657	WP_212608839.1	lasso peptide biosynthesis B2 protein	-
KFK14_RS19440 (KFK14_19440)	3932628..3934328	+	1701	WP_212608840.1	asparagine synthase C-terminal domain-containing protein	-
KFK14_RS19445 (KFK14_19445)	3934455..3934637	+	183	WP_212608841.1	hypothetical protein	-
KFK14_RS19450 (KFK14_19450)	3934686..3934961	-	276	WP_212608842.1	HU family DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (2-131)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 145 a.a.
• Molecular weight: 15343.57 Da
• Isoelectric Point: 6.7640

>T200526 WP_212608836.1 NZ_CP073910:c3931256-3930822 [Sphingobium phenoxybenzoativorans]
VTFLLDVNVLIALIDPYHVGHEAAHHWFEAEGSASWASCPITQNGLIRIIGHPKYPNSPGSPASIAPILRSLCDLPGHIF
WPDAIDITASGTIDIAKILTHAQVTDTYLLALAAHHGGQLATFDRRLATAAVSGGRKALRVIEG

Nucleotide

• Length: 435 bp

>T200526 NZ_CP097884:c2416655-2416281 [Escherichia coli str. K-12 substr. MG1655]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8034.34 Da
• Isoelectric Point: 10.7407

>AT200526 WP_212608837.1 NZ_CP073910:c3931477-3931253 [Sphingobium phenoxybenzoativorans]
MRTTLAIDDDVLLAAKAIAHQQNRTLGEVVSELARKSLRRPASAKSRNGIPLLRADAGKLVTLEMVNALRDEAL

Nucleotide

• Length: 225 bp

>AT200526 NZ_CP097884:c2417112-2416744 [Escherichia coli str. K-12 substr. MG1655]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA