Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | /RES-TIGR02293 |
| Location | 52901..53811 | Replicon | plasmid pHK31_2 |
| Accession | NZ_CP073908 | ||
| Organism | Klebsiella pneumoniae strain HK31 | ||
Toxin (Protein)
| Gene name | - | Uniprot ID | - |
| Locus tag | KFK82_RS27725 | Protein ID | WP_032413375.1 |
| Coordinates | 53341..53811 (+) | Length | 157 a.a. |
Antitoxin (Protein)
| Gene name | - | Uniprot ID | A0A7H4PRG4 |
| Locus tag | KFK82_RS27720 | Protein ID | WP_032413443.1 |
| Coordinates | 52901..53344 (+) | Length | 148 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| KFK82_RS27690 (KFK82_27690) | 48267..48830 | + | 564 | WP_212733079.1 | methyltransferase | - |
| KFK82_RS27695 (KFK82_27695) | 49723..50019 | + | 297 | Protein_57 | single-stranded DNA-binding protein | - |
| KFK82_RS27700 (KFK82_27700) | 50244..50492 | + | 249 | WP_212733080.1 | DUF905 domain-containing protein | - |
| KFK82_RS27705 (KFK82_27705) | 50561..51866 | + | 1306 | Protein_59 | ParB/RepB/Spo0J family partition protein | - |
| KFK82_RS28930 | 51897..51983 | - | 87 | Protein_60 | hypothetical protein | - |
| KFK82_RS27710 (KFK82_27710) | 52003..52188 | + | 186 | WP_071571073.1 | DUF2188 domain-containing protein | - |
| KFK82_RS27715 (KFK82_27715) | 52289..52801 | + | 513 | Protein_62 | DUF4113 domain-containing protein | - |
| KFK82_RS27720 (KFK82_27720) | 52901..53344 | + | 444 | WP_032413443.1 | DUF2384 domain-containing protein | Antitoxin |
| KFK82_RS27725 (KFK82_27725) | 53341..53811 | + | 471 | WP_032413375.1 | RES family NAD+ phosphorylase | Toxin |
| KFK82_RS27730 (KFK82_27730) | 53921..54181 | - | 261 | WP_017901409.1 | hypothetical protein | - |
| KFK82_RS27735 (KFK82_27735) | 54753..55934 | - | 1182 | WP_004883380.1 | IS481 family transposase | - |
| KFK82_RS27740 (KFK82_27740) | 56006..56167 | + | 162 | WP_004118124.1 | type I toxin-antitoxin system Hok family toxin | - |
| KFK82_RS27745 (KFK82_27745) | 56866..57138 | + | 273 | WP_212733081.1 | hypothetical protein | - |
| KFK82_RS27750 (KFK82_27750) | 57135..57485 | + | 351 | WP_032445787.1 | hypothetical protein | - |
| KFK82_RS27755 (KFK82_27755) | 57500..57817 | + | 318 | WP_032445789.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | mcr-8 | - | 1..115662 | 115662 | |
| - | flank | IS/Tn | - | - | 54753..55934 | 1181 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 157 a.a. Molecular weight: 17547.79 Da Isoelectric Point: 4.5152
>T200519 WP_032413375.1 NZ_CP073908:53341-53811 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPDNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIAFRFDSRLKPDRK
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPDNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIAFRFDSRLKPDRK
Download Length: 471 bp
>T200519 NZ_CP097884:1340320-1340616 [Escherichia coli str. K-12 substr. MG1655]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA
Antitoxin
Download Length: 148 a.a. Molecular weight: 16508.82 Da Isoelectric Point: 10.3013
>AT200519 WP_032413443.1 NZ_CP073908:52901-53344 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS
Download Length: 444 bp
>AT200519 NZ_CP097884:1340618-1341013 [Escherichia coli str. K-12 substr. MG1655]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|