Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5290193..5290818 Replicon chromosome
Accession NZ_CP073906
Organism Klebsiella pneumoniae strain HK31

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag KFK82_RS25915 Protein ID WP_002882817.1
Coordinates 5290193..5290576 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag KFK82_RS25920 Protein ID WP_004150355.1
Coordinates 5290576..5290818 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFK82_RS25900 (5287559) 5287559..5288461 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
KFK82_RS25905 (5288458) 5288458..5289093 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
KFK82_RS25910 (5289090) 5289090..5290019 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
KFK82_RS25915 (5290193) 5290193..5290576 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
KFK82_RS25920 (5290576) 5290576..5290818 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
KFK82_RS25925 (5291023) 5291023..5291940 + 918 WP_021466676.1 alpha/beta hydrolase -
KFK82_RS25930 (5291954) 5291954..5292895 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
KFK82_RS25935 (5292940) 5292940..5293377 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
KFK82_RS25940 (5293374) 5293374..5294234 - 861 WP_004146232.1 virulence factor BrkB family protein -
KFK82_RS25945 (5294228) 5294228..5294827 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T200515 WP_002882817.1 NZ_CP073906:c5290576-5290193 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T200515 NZ_CP097884:c154732-154559 [Escherichia coli str. K-12 substr. MG1655]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT200515 WP_004150355.1 NZ_CP073906:c5290818-5290576 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT200515 NZ_CP097884:c155056-154760 [Escherichia coli str. K-12 substr. MG1655]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References