Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4047331..4047950 Replicon chromosome
Accession NZ_CP073906
Organism Klebsiella pneumoniae strain HK31

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag KFK82_RS19985 Protein ID WP_002892050.1
Coordinates 4047732..4047950 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag KFK82_RS19980 Protein ID WP_002892066.1
Coordinates 4047331..4047705 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFK82_RS19970 (4042483) 4042483..4043676 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KFK82_RS19975 (4043699) 4043699..4046845 + 3147 WP_212732983.1 multidrug efflux RND transporter permease subunit AcrB -
KFK82_RS19980 (4047331) 4047331..4047705 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
KFK82_RS19985 (4047732) 4047732..4047950 + 219 WP_002892050.1 HHA domain-containing protein Toxin
KFK82_RS19990 (4048113) 4048113..4048679 + 567 WP_002892030.1 maltose O-acetyltransferase -
KFK82_RS19995 (4048651) 4048651..4048791 - 141 WP_004147370.1 hypothetical protein -
KFK82_RS20000 (4048812) 4048812..4049282 + 471 WP_130948794.1 YlaC family protein -
KFK82_RS20005 (4049257) 4049257..4050708 - 1452 WP_004183206.1 PLP-dependent aminotransferase family protein -
KFK82_RS20010 (4050809) 4050809..4051507 + 699 WP_023287311.1 GNAT family protein -
KFK82_RS20015 (4051504) 4051504..4051644 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
KFK82_RS20020 (4051644) 4051644..4051907 - 264 WP_032432663.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T200510 WP_002892050.1 NZ_CP073906:4047732-4047950 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T200510 NZ_CP097883:3977854-3978195 [Escherichia coli str. K-12 substr. MG1655]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCATGTCACCGTCGGTTA
TGCGAGTCGCTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CTGGCACGGCGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGAGGAGAGCGAA
CTGATGCAGTCGCTACGCCAGGTGTGCAAACTGTCGGCGCGTAAACAAAAGCAGGTGCAGGCGTTTATTGGAGTGATTGC
CGGTAAACAGAAAGTCGCGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT200510 WP_002892066.1 NZ_CP073906:4047331-4047705 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT200510 NZ_CP097883:c3977859-3977783 [Escherichia coli str. K-12 substr. MG1655]
AGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References