Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 1472337..1473073 Replicon chromosome
Accession NZ_CP073906
Organism Klebsiella pneumoniae strain HK31

Toxin (Protein)


Gene name tacT Uniprot ID A0A4S4Y1H2
Locus tag KFK82_RS07280 Protein ID WP_032433360.1
Coordinates 1472591..1473073 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag KFK82_RS07275 Protein ID WP_003026799.1
Coordinates 1472337..1472603 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFK82_RS07250 (1467983) 1467983..1469122 + 1140 WP_032433368.1 mannitol dehydrogenase -
KFK82_RS07255 (1469151) 1469151..1469813 + 663 WP_032433366.1 dihydroxyacetone kinase subunit DhaL -
KFK82_RS07260 (1469797) 1469797..1470801 + 1005 WP_032433364.1 dihydroxyacetone kinase subunit DhaK -
KFK82_RS07265 (1470819) 1470819..1471451 + 633 WP_032433362.1 3-hexulose-6-phosphate synthase -
KFK82_RS07270 (1471461) 1471461..1472024 + 564 WP_032433361.1 6-phospho-3-hexuloisomerase -
KFK82_RS07275 (1472337) 1472337..1472603 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
KFK82_RS07280 (1472591) 1472591..1473073 + 483 WP_032433360.1 GNAT family N-acetyltransferase Toxin
KFK82_RS07285 (1473434) 1473434..1474033 - 600 WP_032432064.1 helix-turn-helix transcriptional regulator -
KFK82_RS07290 (1474246) 1474246..1475190 - 945 WP_077254249.1 fimbrial protein -
KFK82_RS07295 (1475202) 1475202..1475780 - 579 WP_032432061.1 type 1 fimbrial protein -
KFK82_RS07300 (1475784) 1475784..1476524 - 741 WP_050597964.1 molecular chaperone -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1439217..1484016 44799


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(72-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17421.02 Da        Isoelectric Point: 9.6047

>T200500 WP_032433360.1 NZ_CP073906:1472591-1473073 [Klebsiella pneumoniae]
VGRVTAPEPLSSSHQLAEFVSGETVLDEWLKHRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTETTGRLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYTHHGFTASQTHERTLFLKLP

Download         Length: 483 bp

>T200500 NZ_CP097883:2222365-2222652 [Escherichia coli str. K-12 substr. MG1655]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT200500 WP_003026799.1 NZ_CP073906:1472337-1472603 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT200500 NZ_CP097883:2222126-2222365 [Escherichia coli str. K-12 substr. MG1655]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4S4Y1H2

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References