Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 1454579..1455222 Replicon chromosome
Accession NZ_CP073906
Organism Klebsiella pneumoniae strain HK31

Toxin (Protein)


Gene name vagD Uniprot ID A0A4S8C081
Locus tag KFK82_RS07175 Protein ID WP_032433387.1
Coordinates 1454806..1455222 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag KFK82_RS07170 Protein ID WP_001261276.1
Coordinates 1454579..1454809 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFK82_RS07155 (1449601) 1449601..1450688 + 1088 Protein_1398 transcriptional regulator -
KFK82_RS07160 (1450691) 1450691..1452931 + 2241 WP_045326788.1 P-loop NTPase fold protein -
KFK82_RS07165 (1453459) 1453459..1454274 - 816 WP_032433388.1 hypothetical protein -
KFK82_RS07170 (1454579) 1454579..1454809 + 231 WP_001261276.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
KFK82_RS07175 (1454806) 1454806..1455222 + 417 WP_032433387.1 type II toxin-antitoxin system VapC family toxin Toxin
KFK82_RS07180 (1455378) 1455378..1456358 + 981 WP_032433385.1 hypothetical protein -
KFK82_RS07185 (1456553) 1456553..1458124 - 1572 WP_032433383.1 AAA family ATPase -
KFK82_RS07190 (1458443) 1458443..1458691 + 249 WP_032433382.1 hypothetical protein -
KFK82_RS07195 (1458750) 1458750..1459268 + 519 WP_045326794.1 hypothetical protein -
KFK82_RS07200 (1459299) 1459299..1459790 + 492 WP_032433378.1 hypothetical protein -
KFK82_RS07205 (1459850) 1459850..1460053 + 204 WP_212733027.1 hemolysin expression modulator Hha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1439217..1484016 44799


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14974.43 Da        Isoelectric Point: 7.8921

>T200499 WP_032433387.1 NZ_CP073906:1454806-1455222 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALHLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFARVPGLTLEDWVI

Download         Length: 417 bp

>T200499 NZ_CP097883:1944791-1944894 [Escherichia coli str. K-12 substr. MG1655]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT200499 WP_001261276.1 NZ_CP073906:1454579-1454809 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT200499 NZ_CP097883:c1944932-1944661 [Escherichia coli str. K-12 substr. MG1655]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4S8C081

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References