Detailed information of TA system TA200486

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsaAT/-
• Location: 467397..467867
• Replicon: chromosome
• Accession: NZ_CP073900
• Organism: Staphylococcus epidermidis strain V1949610

Toxin (Protein)

• Gene name: TsaT
• Uniprot ID: Q5HQS5
• Locus tag: KFV25_RS02275
• Protein ID: WP_001829569.1
• Coordinates: 467397..467585 (-)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: TsaA
• Uniprot ID: D4FNP2
• Locus tag: KFV25_RS13310
• Protein ID: WP_002438963.1
• Coordinates: 467610..467867 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFV25_RS02245 (KFV25_02245)	463878..464078	+	201	WP_001829681.1	cold-shock protein	-
KFV25_RS02250 (KFV25_02250)	464699..464923	-	225	WP_001829613.1	hypothetical protein	-
KFV25_RS02255 (KFV25_02255)	464947..465231	-	285	WP_001829635.1	hypothetical protein	-
KFV25_RS02260 (KFV25_02260)	465330..466076	-	747	WP_002438959.1	poly-gamma-glutamate hydrolase family protein	-
KFV25_RS02265 (KFV25_02265)	466360..466593	+	234	WP_001829664.1	hypothetical protein	-
KFV25_RS02270 (KFV25_02270)	466653..467207	-	555	WP_001829566.1	lipoprotein N-acylation protein LnsA	-
KFV25_RS02275 (KFV25_02275)	467397..467585	-	189	WP_001829569.1	hypothetical protein	Toxin
KFV25_RS13310	467610..467867	-	258	WP_002438963.1	hypothetical protein	Antitoxin
KFV25_RS02280 (KFV25_02280)	468276..468479	+	204	WP_002438966.1	sterile alpha motif-like domain-containing protein	-
KFV25_RS02285 (KFV25_02285)	468471..468707	-	237	WP_002457224.1	hypothetical protein	-
KFV25_RS02290 (KFV25_02290)	468921..469508	+	588	WP_001829615.1	histidine phosphatase family protein	-
KFV25_RS02295 (KFV25_02295)	469779..470279	-	501	WP_001829623.1	GNAT family N-acetyltransferase	-
KFV25_RS02300 (KFV25_02300)	470865..471020	+	156	WP_001829648.1	hypothetical protein	-
KFV25_RS02305 (KFV25_02305)	471204..471626	-	423	WP_002457221.1	organic hydroperoxide resistance protein	-
KFV25_RS02310 (KFV25_02310)	471787..472503	+	717	WP_001831974.1	type I 3-dehydroquinate dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	470865..471020	155

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7229.94 Da
• Isoelectric Point: 9.7844

>T200486 WP_001829569.1 NZ_CP073900:c467585-467397 [Staphylococcus epidermidis]
MSLHFQILLWLSILFIIAGTILLVTMLKTKKEERKESYLGFTVIFLIFGFAILIYTFIFGIL

Nucleotide

• Length: 189 bp

>T200486 NZ_CP097882:c4109002-4108652 [Escherichia coli str. K-12 substr. MG1655]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 10014.05 Da
• Isoelectric Point: 4.5440

>AT200486 WP_002438963.1 NZ_CP073900:c467867-467610 [Staphylococcus epidermidis]
MKLNKANIFNLIFTILFFSFNILITYNANIDYKLWLIPGLAICGFALFSSLTLVIIYSDLFSEILFFINIILALYYIYPI
FYEFV

Nucleotide

• Length: 258 bp

>AT200486 NZ_CP097882:c4109247-4108996 [Escherichia coli str. K-12 substr. MG1655]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: A0A0N0LUH8

Antitoxin

• AlphaFold DB: A0A0U5IVM3