Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2086900..2087429 Replicon chromosome
Accession NZ_CP073898
Organism Staphylococcus epidermidis strain V1939586

Toxin (Protein)


Gene name mazF Uniprot ID Q5HME7
Locus tag KFV32_RS10005 Protein ID WP_001829891.1
Coordinates 2086900..2087262 (-) Length 121 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID Q5HME6
Locus tag KFV32_RS10010 Protein ID WP_001829931.1
Coordinates 2087259..2087429 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFV32_RS09985 (KFV32_09995) 2083901..2084671 - 771 WP_002468952.1 RNA polymerase sigma factor SigB -
KFV32_RS09990 (KFV32_10000) 2084685..2085125 - 441 WP_063278490.1 anti-sigma B factor RsbW -
KFV32_RS09995 (KFV32_10005) 2085127..2085453 - 327 WP_001829952.1 anti-sigma factor antagonist -
KFV32_RS10000 (KFV32_10010) 2085553..2086554 - 1002 WP_001829902.1 PP2C family protein-serine/threonine phosphatase -
KFV32_RS10005 (KFV32_10015) 2086900..2087262 - 363 WP_001829891.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
KFV32_RS10010 (KFV32_10020) 2087259..2087429 - 171 WP_001829931.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
KFV32_RS10015 (KFV32_10025) 2087516..2088664 - 1149 WP_002468951.1 alanine racemase -
KFV32_RS10020 (KFV32_10030) 2088730..2089083 - 354 WP_001829915.1 holo-ACP synthase -
KFV32_RS10025 (KFV32_10035) 2089131..2089640 - 510 WP_001829888.1 PH domain-containing protein -
KFV32_RS10030 (KFV32_10040) 2089627..2091132 - 1506 WP_001829976.1 PH domain-containing protein -
KFV32_RS10035 (KFV32_10045) 2091125..2091604 - 480 WP_063278489.1 PH domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13497.65 Da        Isoelectric Point: 9.9522

>T200483 WP_001829891.1 NZ_CP073898:c2087262-2086900 [Staphylococcus epidermidis]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTFLSESKMIEVDNALDISLGLNNFDHHKS

Download         Length: 363 bp

>T200483 NZ_CP097882:3669804-3670082 [Escherichia coli str. K-12 substr. MG1655]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6289.92 Da        Isoelectric Point: 4.1920

>AT200483 WP_001829931.1 NZ_CP073898:c2087429-2087259 [Staphylococcus epidermidis]
MLSFNQNRNHSLEQSLKEGYAQMADLNLSLATEAFPIECEACDCNESHLISNSKNE

Download         Length: 171 bp

>AT200483 NZ_CP097882:3669541-3669801 [Escherichia coli str. K-12 substr. MG1655]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2G7HWR0

Antitoxin

Source ID Structure
AlphaFold DB A0A0N1EF65

References