Detailed information of TA system TA200461

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsaAT/-
• Location: 466248..466718
• Replicon: chromosome
• Accession: NZ_CP073887
• Organism: Staphylococcus epidermidis strain V1933625

Toxin (Protein)

• Gene name: TsaT
• Uniprot ID: Q5HQS5
• Locus tag: KFV30_RS02350
• Protein ID: WP_001829569.1
• Coordinates: 466248..466436 (-)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: TsaA
• Uniprot ID: D4FNP2
• Locus tag: KFV30_RS13350
• Protein ID: WP_002438963.1
• Coordinates: 466461..466718 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFV30_RS02320 (KFV30_02320)	462729..462929	+	201	WP_001829681.1	cold-shock protein	-
KFV30_RS02325 (KFV30_02325)	463550..463774	-	225	WP_001829613.1	hypothetical protein	-
KFV30_RS02330 (KFV30_02330)	463798..464082	-	285	WP_001829635.1	hypothetical protein	-
KFV30_RS02335 (KFV30_02335)	464181..464927	-	747	WP_002438959.1	poly-gamma-glutamate hydrolase family protein	-
KFV30_RS02340 (KFV30_02340)	465211..465444	+	234	WP_001829664.1	hypothetical protein	-
KFV30_RS02345 (KFV30_02345)	465504..466058	-	555	WP_001829566.1	lipoprotein N-acylation protein LnsA	-
KFV30_RS02350 (KFV30_02350)	466248..466436	-	189	WP_001829569.1	hypothetical protein	Toxin
KFV30_RS13350	466461..466718	-	258	WP_002438963.1	hypothetical protein	Antitoxin
KFV30_RS02355 (KFV30_02355)	467127..467330	+	204	WP_002438966.1	sterile alpha motif-like domain-containing protein	-
KFV30_RS02360 (KFV30_02360)	467322..467558	-	237	WP_002457224.1	hypothetical protein	-
KFV30_RS02365 (KFV30_02365)	467772..468359	+	588	WP_001829615.1	histidine phosphatase family protein	-
KFV30_RS02370 (KFV30_02370)	468630..469130	-	501	WP_001829623.1	GNAT family N-acetyltransferase	-
KFV30_RS02375 (KFV30_02375)	469734..469871	+	138	WP_162161215.1	hypothetical protein	-
KFV30_RS02380 (KFV30_02380)	470055..470477	-	423	WP_002457221.1	organic hydroperoxide resistance protein	-
KFV30_RS02385 (KFV30_02385)	470638..471354	+	717	WP_001831974.1	type I 3-dehydroquinate dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	448036..475746	27710
	-	flank	IS/Tn	-	-	469716..469871	155

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin GhoT/OrtT (4-57)

Antitoxin

• Endoribonuclease antitoxin GhoS (5-94)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7229.94 Da
• Isoelectric Point: 9.7844

>T200461 WP_001829569.1 NZ_CP073887:c466436-466248 [Staphylococcus epidermidis]
MSLHFQILLWLSILFIIAGTILLVTMLKTKKEERKESYLGFTVIFLIFGFAILIYTFIFGIL

Nucleotide

• Length: 189 bp

>T200461 NZ_CP097881:c4133987-4133814 [Escherichia coli str. K-12 substr. MG1655]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 10014.05 Da
• Isoelectric Point: 4.5440

>AT200461 WP_002438963.1 NZ_CP073887:c466718-466461 [Staphylococcus epidermidis]
MKLNKANIFNLIFTILFFSFNILITYNANIDYKLWLIPGLAICGFALFSSLTLVIIYSDLFSEILFFINIILALYYIYPI
FYEFV

Nucleotide

• Length: 258 bp

>AT200461 NZ_CP097881:c4134311-4134015 [Escherichia coli str. K-12 substr. MG1655]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: A0A0N0LUH8

Antitoxin

• AlphaFold DB: A0A0U5IVM3