Detailed information of TA system TA020045

Bioinformatically predicted

Overview

TA module

• Type: V
• Classification (family/domain): ghoTS/-
• Location: 4324814..4325311
• Replicon: chromosome
• Accession: CP009789
• Organism: Escherichia coli K-12 strain ER3413

Toxin (Protein)

• Gene name: ghoT
• Uniprot ID: -
• Locus tag: ER3413_4272
• Protein ID: AIZ53945.1
• Coordinates: 4325138..4325311 (+)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: ghoS
• Uniprot ID: -
• Locus tag: ER3413_4271
• Protein ID: AIZ53944.1
• Coordinates: 4324814..4325110 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ER3413_4266	4321545..4322264	-	720	AIZ53939.1	DNA-binding response regulator in two-component regulatory system with DcuS	-
ER3413_4267	4322261..4323892	-	1632	AIZ53940.1	sensory histidine kinase in two-component regulatory system with DcuR, regulator of anaerobic fumarate respiration	-
ER3413_4269	4324073..4324303	+	231	AIZ53941.1	hypothetical protein	-
ER3413_4270	4324315..4324587	+	273	AIZ53943.1	putative acyltransferase with acyl-CoA N-acyltransferase domain	-
ER3413_4271	4324814..4325110	+	297	AIZ53944.1	hypothetical protein	Antitoxin
ER3413_4272	4325138..4325311	+	174	AIZ53945.1	hypothetical protein	Toxin
ER3413_4273	4325430..4326947	-	1518	AIZ53946.1	lysine tRNA synthetase, inducible	-
ER3413_4274	4327184..4328641	-	1458	AIZ53947.1	dipeptide and tripeptide permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin GhoT/OrtT (4-57)

Antitoxin

• Endoribonuclease antitoxin GhoS (5-94)

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6555.06 Da
• Isoelectric Point: 10.2288

>T20045 AIZ53945.1 CP009789:4325138-4325311 [Escherichia coli K-12]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Nucleotide

• Length: 174 bp

>T20045 CP009789:4325138-4325311 [Escherichia coli K-12]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11467.97 Da
• Isoelectric Point: 4.1705

>AT20045 AIZ53944.1 CP009789:4324814-4325110 [Escherichia coli K-12]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Nucleotide

• Length: 297 bp

>AT20045 CP009789:4324814-4325110 [Escherichia coli K-12]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG