Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 693031..693560 Replicon chromosome
Accession NZ_CP073878
Organism Staphylococcus epidermidis strain B1285228

Toxin (Protein)


Gene name mazF Uniprot ID Q5HME7
Locus tag KFV29_RS03680 Protein ID WP_001829891.1
Coordinates 693198..693560 (+) Length 121 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID Q5HME6
Locus tag KFV29_RS03675 Protein ID WP_001829931.1
Coordinates 693031..693201 (+) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFV29_RS03650 (KFV29_03650) 688856..689335 + 480 WP_063278489.1 PH domain-containing protein -
KFV29_RS03655 (KFV29_03655) 689328..690833 + 1506 WP_001829976.1 PH domain-containing protein -
KFV29_RS03660 (KFV29_03660) 690820..691329 + 510 WP_001829888.1 PH domain-containing protein -
KFV29_RS03665 (KFV29_03665) 691377..691730 + 354 WP_001829915.1 holo-ACP synthase -
KFV29_RS03670 (KFV29_03670) 691796..692944 + 1149 WP_002468951.1 alanine racemase -
KFV29_RS03675 (KFV29_03675) 693031..693201 + 171 WP_001829931.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
KFV29_RS03680 (KFV29_03680) 693198..693560 + 363 WP_001829891.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
KFV29_RS03685 (KFV29_03685) 693906..694907 + 1002 WP_001829902.1 PP2C family protein-serine/threonine phosphatase -
KFV29_RS03690 (KFV29_03690) 695007..695333 + 327 WP_001829952.1 anti-sigma factor antagonist -
KFV29_RS03695 (KFV29_03695) 695335..695775 + 441 WP_063278490.1 anti-sigma B factor RsbW -
KFV29_RS03700 (KFV29_03700) 695789..696559 + 771 WP_002468952.1 RNA polymerase sigma factor SigB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13497.65 Da        Isoelectric Point: 9.9522

>T200448 WP_001829891.1 NZ_CP073878:693198-693560 [Staphylococcus epidermidis]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTFLSESKMIEVDNALDISLGLNNFDHHKS

Download         Length: 363 bp

>T200448 NZ_CP097881:2237318-2237605 [Escherichia coli str. K-12 substr. MG1655]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6289.92 Da        Isoelectric Point: 4.1920

>AT200448 WP_001829931.1 NZ_CP073878:693031-693201 [Staphylococcus epidermidis]
MLSFNQNRNHSLEQSLKEGYAQMADLNLSLATEAFPIECEACDCNESHLISNSKNE

Download         Length: 171 bp

>AT200448 NZ_CP097881:2237079-2237318 [Escherichia coli str. K-12 substr. MG1655]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2G7HWR0

Antitoxin

Source ID Structure
AlphaFold DB A0A0N1EF65

References