Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2019739..2020268 Replicon chromosome
Accession NZ_CP073876
Organism Staphylococcus epidermidis strain B1285135

Toxin (Protein)


Gene name mazF Uniprot ID Q5HME7
Locus tag KFV37_RS09745 Protein ID WP_001829891.1
Coordinates 2019739..2020101 (-) Length 121 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID Q5HME6
Locus tag KFV37_RS09750 Protein ID WP_001829931.1
Coordinates 2020098..2020268 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFV37_RS09725 (KFV37_09765) 2016740..2017510 - 771 WP_002468952.1 RNA polymerase sigma factor SigB -
KFV37_RS09730 (KFV37_09770) 2017524..2017964 - 441 WP_063278490.1 anti-sigma B factor RsbW -
KFV37_RS09735 (KFV37_09775) 2017966..2018292 - 327 WP_001829952.1 anti-sigma factor antagonist -
KFV37_RS09740 (KFV37_09780) 2018392..2019393 - 1002 WP_001829902.1 PP2C family protein-serine/threonine phosphatase -
KFV37_RS09745 (KFV37_09785) 2019739..2020101 - 363 WP_001829891.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
KFV37_RS09750 (KFV37_09790) 2020098..2020268 - 171 WP_001829931.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
KFV37_RS09755 (KFV37_09795) 2020355..2021503 - 1149 WP_002468951.1 alanine racemase -
KFV37_RS09760 (KFV37_09800) 2021569..2021922 - 354 WP_001829915.1 holo-ACP synthase -
KFV37_RS09765 (KFV37_09805) 2021970..2022479 - 510 WP_001829888.1 PH domain-containing protein -
KFV37_RS09770 (KFV37_09810) 2022466..2023971 - 1506 WP_001829976.1 PH domain-containing protein -
KFV37_RS09775 (KFV37_09815) 2023964..2024443 - 480 WP_063278489.1 PH domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13497.65 Da        Isoelectric Point: 9.9522

>T200443 WP_001829891.1 NZ_CP073876:c2020101-2019739 [Staphylococcus epidermidis]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTFLSESKMIEVDNALDISLGLNNFDHHKS

Download         Length: 363 bp

>T200443 NZ_CP097881:c1117811-1116738 [Escherichia coli str. K-12 substr. MG1655]
ATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGCAAATATCGAGACTGAATCCAGACAATTCATTGAAAATAA
AAACTATTCAATCCAATCAATTGGTCCTATGCCAGGGTCAAGGGCTGGGCTTCGGGTCGTATTTACCAGACCAGGGGTTA
ACTTGGCAACTGTGGACATTTTTTATAACGGGGACGGTTCGACTACAATTCAATATCTCACTGGAGCCAATCGTTCTCTG
GGCCAAGAGTTAGCGGATCATCTTTTTGAAACCATCAATCCTGCTGAATTTGAGCAGGTAAATATGGTACTGCAAGGATT
TGTAGAGACAAGCGTTCTACCTGTACTTGAGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACG
CTCATACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCATATCACAACAGGTAAG
CTCCAGATTCAGGGCCGACCGCTGTCATGTTACAGAGTTTTCACGTTTAACTTGGCAGCCCTGCTTGATTTACAGGGTTT
GGAGAAAGTGCTAATCCGCCAGGAGGATGGTAAAGCTAATATTGTTCAACAGGAGGTTGCCCGCACTTACTTGCAGACTG
TAATGGCCGATGCTTACCCGCATCTCCACGTGACTGCCGAAAAATTGCTCGTTTCAGGGCTATGTGTTAAACTCGCCGCC
CCTGATTTGCCTGACTACTGTATGTTACTTTATCCTGAACTACGCACCATTGAAGGTGTCTTAAAAAGTAAGATGAGTGG
GTTAGGCATGCCAGTACAGCAGCCGGCAGGTTTTGGAACTTACTTTGATAAACCTGCTGCTCATTACATTCTGAAACCGC
AATTTGCAGCTACTCTTAGACCGGAACAGATTAACATCATCAGCACAGCCTATACTTTTTTTAATGTGGAACGTCATTCT
CTGTTCCACATGGAAACTGTGGTCGATGCCAGCCGTATGATTTCTGATATGGCCCGGTTGATGGGTAAAGCCACTAGAGC
GTGGGGAATAATCAAGGACTTATATATTGTTTGA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6289.92 Da        Isoelectric Point: 4.1920

>AT200443 WP_001829931.1 NZ_CP073876:c2020268-2020098 [Staphylococcus epidermidis]
MLSFNQNRNHSLEQSLKEGYAQMADLNLSLATEAFPIECEACDCNESHLISNSKNE

Download         Length: 171 bp

>AT200443 NZ_CP097881:c1116745-1116374 [Escherichia coli str. K-12 substr. MG1655]
TTGTTTGAAATCACCGGAATTAACGTCTCAGGGGCTCTAAAAGCTGTCGTTATGGCGACCGGCTTCGAGAACCCTTTGTC
CTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCTTGT
GCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATT
ATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAACTGACTTTTACTTTACGTAATCATCCTGAGTACCTAGAAGC
CTCTGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2G7HWR0

Antitoxin

Source ID Structure
AlphaFold DB A0A0N1EF65

References