Detailed information of TA system TA200438

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2022366..2022895
• Replicon: chromosome
• Accession: NZ_CP073872
• Organism: Staphylococcus epidermidis strain B1282731

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: Q5HME7
• Locus tag: KFV34_RS09755
• Protein ID: WP_001829891.1
• Coordinates: 2022366..2022728 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: Q5HME6
• Locus tag: KFV34_RS09760
• Protein ID: WP_001829931.1
• Coordinates: 2022725..2022895 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFV34_RS09735 (KFV34_09745)	2019367..2020137	-	771	WP_002468952.1	RNA polymerase sigma factor SigB	-
KFV34_RS09740 (KFV34_09750)	2020151..2020591	-	441	WP_063278490.1	anti-sigma B factor RsbW	-
KFV34_RS09745 (KFV34_09755)	2020593..2020919	-	327	WP_001829952.1	anti-sigma factor antagonist	-
KFV34_RS09750 (KFV34_09760)	2021019..2022020	-	1002	WP_001829902.1	PP2C family protein-serine/threonine phosphatase	-
KFV34_RS09755 (KFV34_09765)	2022366..2022728	-	363	WP_001829891.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KFV34_RS09760 (KFV34_09770)	2022725..2022895	-	171	WP_001829931.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
KFV34_RS09765 (KFV34_09775)	2022982..2024130	-	1149	WP_002468951.1	alanine racemase	-
KFV34_RS09770 (KFV34_09780)	2024196..2024549	-	354	WP_001829915.1	holo-ACP synthase	-
KFV34_RS09775 (KFV34_09785)	2024597..2025106	-	510	WP_001829888.1	PH domain-containing protein	-
KFV34_RS09780 (KFV34_09790)	2025093..2026598	-	1506	WP_001829976.1	PH domain-containing protein	-
KFV34_RS09785 (KFV34_09795)	2026591..2027070	-	480	WP_063278489.1	PH domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13497.65 Da
• Isoelectric Point: 9.9522

>T200438 WP_001829891.1 NZ_CP073872:c2022728-2022366 [Staphylococcus epidermidis]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTFLSESKMIEVDNALDISLGLNNFDHHKS

Nucleotide

• Length: 363 bp

>T200438 NZ_CP097881:649276-649590 [Escherichia coli str. K-12 substr. MG1655]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6289.92 Da
• Isoelectric Point: 4.1920

>AT200438 WP_001829931.1 NZ_CP073872:c2022895-2022725 [Staphylococcus epidermidis]
MLSFNQNRNHSLEQSLKEGYAQMADLNLSLATEAFPIECEACDCNESHLISNSKNE

Nucleotide

• Length: 171 bp

>AT200438 NZ_CP097881:649587-650003 [Escherichia coli str. K-12 substr. MG1655]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Toxin

• AlphaFold DB: A0A2G7HWR0

Antitoxin

• AlphaFold DB: A0A0N1EF65