Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2022366..2022895 Replicon chromosome
Accession NZ_CP073872
Organism Staphylococcus epidermidis strain B1282731

Toxin (Protein)


Gene name mazF Uniprot ID Q5HME7
Locus tag KFV34_RS09755 Protein ID WP_001829891.1
Coordinates 2022366..2022728 (-) Length 121 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID Q5HME6
Locus tag KFV34_RS09760 Protein ID WP_001829931.1
Coordinates 2022725..2022895 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFV34_RS09735 (KFV34_09745) 2019367..2020137 - 771 WP_002468952.1 RNA polymerase sigma factor SigB -
KFV34_RS09740 (KFV34_09750) 2020151..2020591 - 441 WP_063278490.1 anti-sigma B factor RsbW -
KFV34_RS09745 (KFV34_09755) 2020593..2020919 - 327 WP_001829952.1 anti-sigma factor antagonist -
KFV34_RS09750 (KFV34_09760) 2021019..2022020 - 1002 WP_001829902.1 PP2C family protein-serine/threonine phosphatase -
KFV34_RS09755 (KFV34_09765) 2022366..2022728 - 363 WP_001829891.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
KFV34_RS09760 (KFV34_09770) 2022725..2022895 - 171 WP_001829931.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
KFV34_RS09765 (KFV34_09775) 2022982..2024130 - 1149 WP_002468951.1 alanine racemase -
KFV34_RS09770 (KFV34_09780) 2024196..2024549 - 354 WP_001829915.1 holo-ACP synthase -
KFV34_RS09775 (KFV34_09785) 2024597..2025106 - 510 WP_001829888.1 PH domain-containing protein -
KFV34_RS09780 (KFV34_09790) 2025093..2026598 - 1506 WP_001829976.1 PH domain-containing protein -
KFV34_RS09785 (KFV34_09795) 2026591..2027070 - 480 WP_063278489.1 PH domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13497.65 Da        Isoelectric Point: 9.9522

>T200438 WP_001829891.1 NZ_CP073872:c2022728-2022366 [Staphylococcus epidermidis]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTFLSESKMIEVDNALDISLGLNNFDHHKS

Download         Length: 363 bp

>T200438 NZ_CP097881:649276-649590 [Escherichia coli str. K-12 substr. MG1655]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6289.92 Da        Isoelectric Point: 4.1920

>AT200438 WP_001829931.1 NZ_CP073872:c2022895-2022725 [Staphylococcus epidermidis]
MLSFNQNRNHSLEQSLKEGYAQMADLNLSLATEAFPIECEACDCNESHLISNSKNE

Download         Length: 171 bp

>AT200438 NZ_CP097881:649587-650003 [Escherichia coli str. K-12 substr. MG1655]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2G7HWR0

Antitoxin

Source ID Structure
AlphaFold DB A0A0N1EF65

References