Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2022206..2022735 Replicon chromosome
Accession NZ_CP073869
Organism Staphylococcus epidermidis strain B1279591

Toxin (Protein)


Gene name mazF Uniprot ID Q5HME7
Locus tag KFV51_RS09750 Protein ID WP_001829891.1
Coordinates 2022206..2022568 (-) Length 121 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID Q5HME6
Locus tag KFV51_RS09755 Protein ID WP_001829931.1
Coordinates 2022565..2022735 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFV51_RS09730 (KFV51_09770) 2019207..2019977 - 771 WP_002468952.1 RNA polymerase sigma factor SigB -
KFV51_RS09735 (KFV51_09775) 2019991..2020431 - 441 WP_063278490.1 anti-sigma B factor RsbW -
KFV51_RS09740 (KFV51_09780) 2020433..2020759 - 327 WP_001829952.1 anti-sigma factor antagonist -
KFV51_RS09745 (KFV51_09785) 2020859..2021860 - 1002 WP_001829902.1 PP2C family protein-serine/threonine phosphatase -
KFV51_RS09750 (KFV51_09790) 2022206..2022568 - 363 WP_001829891.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
KFV51_RS09755 (KFV51_09795) 2022565..2022735 - 171 WP_001829931.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
KFV51_RS09760 (KFV51_09800) 2022822..2023970 - 1149 WP_002468951.1 alanine racemase -
KFV51_RS09765 (KFV51_09805) 2024036..2024389 - 354 WP_001829915.1 holo-ACP synthase -
KFV51_RS09770 (KFV51_09810) 2024437..2024946 - 510 WP_001829888.1 PH domain-containing protein -
KFV51_RS09775 (KFV51_09815) 2024933..2026438 - 1506 WP_001829976.1 PH domain-containing protein -
KFV51_RS09780 (KFV51_09820) 2026431..2026910 - 480 WP_063278489.1 PH domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13497.65 Da        Isoelectric Point: 9.9522

>T200433 WP_001829891.1 NZ_CP073869:c2022568-2022206 [Staphylococcus epidermidis]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTFLSESKMIEVDNALDISLGLNNFDHHKS

Download         Length: 363 bp

>T200433 NZ_CP097870:3707222-3707623 [Thauera sp. GDN1]
ATGCTGCGCTACCTGCTCGACACCAACATCGTGATCTACGTGATCAAGCGCCGGCCGCTGTCGGCGCTGGCCTTGTTCAA
CGAAAACGCCGGCCACATGGCGATCTCGTCGATCACCCTCGCGGAGTTGATGCACGGCGCTGAAGAGAGCAACGCGCCGA
CGCGCTCACTGGCCGCAGTCGAGGATTTCTGCAGCCGCCTTGAAGTGCTGCCTTACGGGCCCAAGGCCGCGATGCACTAT
GGAAATATCCGCGCCCATCTCGAATCGCGCGGCCAGACGATCGGCGTCAATGACCTGCACATCGCCGCTCACGCACGCAG
CGAGGGGCTGACGCTGGTGTCGAACAACCTGCGGGAGTTCGAGCGCGTGGAAGCGCTGCAACTGGCGAACTGGGCGATTT
GA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6289.92 Da        Isoelectric Point: 4.1920

>AT200433 WP_001829931.1 NZ_CP073869:c2022735-2022565 [Staphylococcus epidermidis]
MLSFNQNRNHSLEQSLKEGYAQMADLNLSLATEAFPIECEACDCNESHLISNSKNE

Download         Length: 171 bp

>AT200433 NZ_CP097870:3706989-3707222 [Thauera sp. GDN1]
ATGACCGCGAGCACCGTCTTTACCAACAACCGCAGCCAGGCCGTGCGCCTGCCGGCCGACGTGCGCCTGCCCCCCGATGT
GAAGCGGGTGGATATCCGCGCGCGTGGCGTGGAGCGAATCATCGCGCCGGTGGGCCATACTTGGGACTCGTTCTTCATCG
ACGGCCCACAGGTCGCCGACGACTTCATGGAAACCCGCGCCAGCCAGGAACAGCAGGAACGCGAGGCGCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2G7HWR0

Antitoxin

Source ID Structure
AlphaFold DB A0A0N1EF65

References