Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2022347..2022876 Replicon chromosome
Accession NZ_CP073863
Organism Staphylococcus epidermidis strain B1276296

Toxin (Protein)


Gene name mazF Uniprot ID Q5HME7
Locus tag KFV46_RS09670 Protein ID WP_001829891.1
Coordinates 2022347..2022709 (-) Length 121 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID Q5HME6
Locus tag KFV46_RS09675 Protein ID WP_001829931.1
Coordinates 2022706..2022876 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFV46_RS09650 (KFV46_09660) 2019348..2020118 - 771 WP_002468952.1 RNA polymerase sigma factor SigB -
KFV46_RS09655 (KFV46_09665) 2020132..2020572 - 441 WP_063278490.1 anti-sigma B factor RsbW -
KFV46_RS09660 (KFV46_09670) 2020574..2020900 - 327 WP_001829952.1 anti-sigma factor antagonist -
KFV46_RS09665 (KFV46_09675) 2021000..2022001 - 1002 WP_001829902.1 PP2C family protein-serine/threonine phosphatase -
KFV46_RS09670 (KFV46_09680) 2022347..2022709 - 363 WP_001829891.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
KFV46_RS09675 (KFV46_09685) 2022706..2022876 - 171 WP_001829931.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
KFV46_RS09680 (KFV46_09690) 2022963..2024111 - 1149 WP_002468951.1 alanine racemase -
KFV46_RS09685 (KFV46_09695) 2024177..2024530 - 354 WP_001829915.1 holo-ACP synthase -
KFV46_RS09690 (KFV46_09700) 2024578..2025087 - 510 WP_001829888.1 PH domain-containing protein -
KFV46_RS09695 (KFV46_09705) 2025074..2026579 - 1506 WP_001829976.1 PH domain-containing protein -
KFV46_RS09700 (KFV46_09710) 2026572..2027051 - 480 WP_063278489.1 PH domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13497.65 Da        Isoelectric Point: 9.9522

>T200423 WP_001829891.1 NZ_CP073863:c2022709-2022347 [Staphylococcus epidermidis]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTFLSESKMIEVDNALDISLGLNNFDHHKS

Download         Length: 363 bp

>T200423 NZ_CP097859:c2094854-2093913 [Vibrio owensii]
ATGAACACACTAAAACAACAGTTCCTTGAGGTTTCCGATGCGCTTGAGCTCGGAAACCCAGCCATTATTGAAAAAGATTA
CTGGGTCGTGGCTTTACTGGCTGAGCTAGCGAAAGTTTGTCCTGAGCATCATCAAATGGTCTTTTCTGGCGGCACTGCGT
TGGCGAAATCAAACGTTAAAATTCTAAGAATGTCGGAAGATGTGGATATAAAACTCATCCCCAACCAAGCATTTGATGAA
CTGAGCCGAGCGAAAAAGAAAGCTGCGAGAAAGGCATGCATAAATGAGATTGAACAGGCGCTCACCCAGTCAGGGCGATT
TAACGTTGAAACGCGCTCTGTGCGAGATGAGTACCGCTACGTCGAGTTTGAACTTAAATACCCGCAGCAGTTTAGCCAAG
CCCCCTGTCTTCGCCCCATCATCAAGTTAGAGTTAATCGAAACTATTCCATTACTGGATGTGGAGTCGCGCAGTATTCAA
TCTCTGGTCGCAGAGTTGTACCAACAACCACAAGAAGTGAGTGCATTTGATTGCGTCTCTATCCATGCCACATTGGTTGA
AAAAATTATTTCAATGCTGCGCCGTACCATGTCGGTAAAACGCAATACGGAGCGAAGCGATGATGTTGCTTTAGTTCGCC
ACATCTACGATGTGCACTGCATAGCAAAACTCTCAATGCCAGAACTCGACTCGATTGACGGTCTATTCAAAGCGGTATTG
GCAGAAGATATTGCCCGCTTTGGCAATCAACATGCCGAGTTTGTTGCCAACCCCAAACAAGAGCTACAACTTGGCCTTAA
AGAGCTTGAGGAAAATGCAATATTCCGCCAACGCTTTCAGGACTTCGTCACGCCAATGGTGTTTAACACCGAACCCCATG
ATTTTGATACCTGCTTTGCCTCATTTAAACGCATTGCAGCATCGCTAATTGAAAAAATTTAA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6289.92 Da        Isoelectric Point: 4.1920

>AT200423 WP_001829931.1 NZ_CP073863:c2022876-2022706 [Staphylococcus epidermidis]
MLSFNQNRNHSLEQSLKEGYAQMADLNLSLATEAFPIECEACDCNESHLISNSKNE

Download         Length: 171 bp

>AT200423 NZ_CP097859:c2095260-2094856 [Vibrio owensii]
ATGACAGCAGTAGACCGCATTTGTCAAAAGATAAAGCGTTCAAGACGTTATGTATTTGAACGCAAAGACTTTGATGGATT
TGCAAGTTACGACCAAATAGGTCGGGCCCTGAGGCAGCTTGTGCTGCAAGGTGAGTTAATGAAGCTTGGCTATGGCTTGT
ATACCAAAGCAAGAATCAATAGCCTCACAGGTAAGCCTATGCCGACTAATCCGGGAGGGACCGATGCCCTTATGCGAGAA
ATTCTTAAAATGAAAGGCGTTGATTTTGAGATGGATAGTCTATCGCTACAAAGTCTTTCAGGCACTAGCACCCAAATTCC
ATCTTCTATTCAGTATTCATGGGACCCAAAACAGTTTAATCGCAAACTGGTGGTTGGTAGCCGTGTGCTGAACTTACCAA
GTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2G7HWR0

Antitoxin

Source ID Structure
AlphaFold DB A0A0N1EF65

References