Detailed information of TA system TA200420

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsaAT/-
• Location: 2245576..2246046
• Replicon: chromosome
• Accession: NZ_CP073862
• Organism: Staphylococcus epidermidis strain B1276219

Toxin (Protein)

• Gene name: TsaT
• Uniprot ID: Q5HQS5
• Locus tag: KFV45_RS11010
• Protein ID: WP_001829569.1
• Coordinates: 2245858..2246046 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: TsaA
• Uniprot ID: D4FNP2
• Locus tag: KFV45_RS13425
• Protein ID: WP_002438963.1
• Coordinates: 2245576..2245833 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFV45_RS10975 (KFV45_10985)	2240940..2241656	-	717	WP_001831974.1	type I 3-dehydroquinate dehydratase	-
KFV45_RS10980 (KFV45_10990)	2241817..2242239	+	423	WP_002457221.1	organic hydroperoxide resistance protein	-
KFV45_RS10985 (KFV45_10995)	2242423..2242560	-	138	WP_162161215.1	hypothetical protein	-
KFV45_RS10990 (KFV45_11000)	2243164..2243664	+	501	WP_001829623.1	GNAT family N-acetyltransferase	-
KFV45_RS10995 (KFV45_11005)	2243935..2244522	-	588	WP_001829615.1	histidine phosphatase family protein	-
KFV45_RS11000 (KFV45_11010)	2244736..2244972	+	237	WP_002457224.1	hypothetical protein	-
KFV45_RS11005 (KFV45_11015)	2244964..2245167	-	204	WP_002438966.1	sterile alpha motif-like domain-containing protein	-
KFV45_RS13425	2245576..2245833	+	258	WP_002438963.1	hypothetical protein	Antitoxin
KFV45_RS11010 (KFV45_11020)	2245858..2246046	+	189	WP_001829569.1	hypothetical protein	Toxin
KFV45_RS11015 (KFV45_11025)	2246236..2246790	+	555	WP_001829566.1	lipoprotein N-acylation protein LnsA	-
KFV45_RS11020 (KFV45_11030)	2246850..2247083	-	234	WP_001829664.1	hypothetical protein	-
KFV45_RS11025 (KFV45_11035)	2247367..2248113	+	747	WP_002438959.1	poly-gamma-glutamate hydrolase family protein	-
KFV45_RS11030 (KFV45_11040)	2248212..2248496	+	285	WP_001829635.1	hypothetical protein	-
KFV45_RS11035 (KFV45_11045)	2248520..2248744	+	225	WP_001829613.1	hypothetical protein	-
KFV45_RS11040 (KFV45_11050)	2249365..2249565	-	201	WP_001829681.1	cold-shock protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2236548..2260832	24284
	-	flank	IS/Tn	-	-	2242423..2242578	155

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (20-69)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7229.94 Da
• Isoelectric Point: 9.7844

>T200420 WP_001829569.1 NZ_CP073862:2245858-2246046 [Staphylococcus epidermidis]
MSLHFQILLWLSILFIIAGTILLVTMLKTKKEERKESYLGFTVIFLIFGFAILIYTFIFGIL

Nucleotide

• Length: 189 bp

>T200420 NZ_CP097857:c6655302-6655024 [Pseudomonas sp. B111]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCATCATGCGGCTACGGAGCTTCGAGACTTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGTGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 10014.05 Da
• Isoelectric Point: 4.5440

>AT200420 WP_002438963.1 NZ_CP073862:2245576-2245833 [Staphylococcus epidermidis]
MKLNKANIFNLIFTILFFSFNILITYNANIDYKLWLIPGLAICGFALFSSLTLVIIYSDLFSEILFFINIILALYYIYPI
FYEFV

Nucleotide

• Length: 258 bp

>AT200420 NZ_CP097857:c6655012-6654707 [Pseudomonas sp. B111]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTACGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCCCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTACTCGTTAGCA
ACTGCTTATGCGGCGAATGGCGAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: A0A0N0LUH8

Antitoxin

• AlphaFold DB: A0A0U5IVM3