Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2022364..2022893 Replicon chromosome
Accession NZ_CP073859
Organism Staphylococcus epidermidis strain B1275857

Toxin (Protein)


Gene name mazF Uniprot ID Q5HME7
Locus tag KFV44_RS09710 Protein ID WP_001829891.1
Coordinates 2022364..2022726 (-) Length 121 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID Q5HME6
Locus tag KFV44_RS09715 Protein ID WP_001829931.1
Coordinates 2022723..2022893 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFV44_RS09690 (KFV44_09700) 2019365..2020135 - 771 WP_002468952.1 RNA polymerase sigma factor SigB -
KFV44_RS09695 (KFV44_09705) 2020149..2020589 - 441 WP_063278490.1 anti-sigma B factor RsbW -
KFV44_RS09700 (KFV44_09710) 2020591..2020917 - 327 WP_001829952.1 anti-sigma factor antagonist -
KFV44_RS09705 (KFV44_09715) 2021017..2022018 - 1002 WP_001829902.1 PP2C family protein-serine/threonine phosphatase -
KFV44_RS09710 (KFV44_09720) 2022364..2022726 - 363 WP_001829891.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
KFV44_RS09715 (KFV44_09725) 2022723..2022893 - 171 WP_001829931.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
KFV44_RS09720 (KFV44_09730) 2022980..2024128 - 1149 WP_002468951.1 alanine racemase -
KFV44_RS09725 (KFV44_09735) 2024194..2024547 - 354 WP_001829915.1 holo-ACP synthase -
KFV44_RS09730 (KFV44_09740) 2024595..2025104 - 510 WP_001829888.1 PH domain-containing protein -
KFV44_RS09735 (KFV44_09745) 2025091..2026596 - 1506 WP_001829976.1 PH domain-containing protein -
KFV44_RS09740 (KFV44_09750) 2026589..2027068 - 480 WP_063278489.1 PH domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13497.65 Da        Isoelectric Point: 9.9522

>T200413 WP_001829891.1 NZ_CP073859:c2022726-2022364 [Staphylococcus epidermidis]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTFLSESKMIEVDNALDISLGLNNFDHHKS

Download         Length: 363 bp

>T200413 NZ_CP097847:c10365-9160 [Neisseria gonorrhoeae]
ATGGTAAAACTGTCTAGCGATATTAATTTGAGAGATTTTGGCAATAACGAATATTTGTCTTCCGTTCAGGATGAGGCCAT
CCGTTTTGCGACCGAACAAACAGACGAGATATTGTCTTTATATAGCCAACATGCAGATACAGAAGGCGGTAGATATGTTT
GTGCTGATACGTTTAAAGAGTTGTTTCCAGCTTTTGAGAACAAAGAAGACAGGGCAACGGTTAATAATGCCATCCACAAT
TCAGCAGCAGTTTTATCTTCTACCCAGTTTGACGAGGTACTGAAACGCGACGAGCCACAGAAGAAAGAAGTTATTTTCGT
TACGGGAATTCCGGGTTCGGGAAAAACTTCGACAGTAAAAAACATGATGATGCAGGATACCACCAAGCTGCTTTTTGAAG
GACAGCTTGCACGGCCGCAGTCTGCTTTTCGCAAGATTGAGCAGTGTCTGGAGCGTAATCTTGAAGTTACTATCGTAGCC
GTCAGCATGAGGGCTGAAAGGGCTTCCGACAATACTTACAAACGGTTTAACGAATACGGCAGGGGAGCCAGTATAGGTAT
TATGGCTGATATTCAGGCCAATCTTCCTGACGGACTCAAGCAAATCAGGGATAAGTTTGGTGATGCGGTAAAAATTGTCG
GTATCAACCAAGACAGGAACAGTGAATTTATCGACAAGTTCGATGATGTCATCAAAATGCTTTCCTTGGGGTCGCAAGAG
CAAATATTGGGCAGGTTGGCCGAGAAAATCCAGAGCGACTTCGACTCAGGAAAAATCAGCCGGGAATGCTTCAATCAGGC
CAAGGGCAGTATGGATCTTGAAAGTGTTTTCGCCAAAAAAGAGTATTCCCAGCAAAGGGTAGTTACTAACTCCAAGGGTG
TTACCCTAGAAACAAAGTCAGCAAACGAGTTGTGGTCTAAGGTGGAACAAATTCCTGTAACAGGCATGAAAGCCGGTATC
TATTTACTAGGTCAAGCTAAAAAAGCAGAGACTGGTCAAACTTATTCCGGAGAAATCATTTACAAGGATGCCGCTGCGGT
ATTTCAAAAAACCAAAAACGGGCTTGTCAGGCACAATGCAACACATAACGAAGAAAGACTGGCCAAATTGGTTGAGATAG
GGCAGAACGTTTCTATCGGAAGCAATAAGGGCAAATTGATTGTCAAGTCTTTGGAGTATTCCGCCAAAAAATCCATCAGC
AGATAA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6289.92 Da        Isoelectric Point: 4.1920

>AT200413 WP_001829931.1 NZ_CP073859:c2022893-2022723 [Staphylococcus epidermidis]
MLSFNQNRNHSLEQSLKEGYAQMADLNLSLATEAFPIECEACDCNESHLISNSKNE

Download         Length: 171 bp

>AT200413 NZ_CP097847:c9145-8714 [Neisseria gonorrhoeae]
ATGAAAGAAAAAATGCGGTTGATCACGGAAATTTCGCTGATTATCAGGGAACTGTCTTATTCCCGGCGCGGGTATCGGCC
TGAGAGTTATGCGGCTGCGCTGGATAATTTGACGATAATCGGAGATTTGGCCGACATTTTGCACAACATCGAGGCAGCGA
CAGGCAATCCATTTGTTCAAAGGATGGTTGATGAAAAACTGAGAAAGTTTGTTACCGATTTTCCGGCTTATAAGGTACGG
TTGTCTCCGTTTTTGAATGGCTGTCCCCATGACCTTTCGTTAGGAGCAGAATCGTTGGGTAAGGGGGAGAGTATCGCTGA
TGCCTTGGCCGTTCCTCCGGAAGAGGTAGATATTGCAGACATTGAGTTTGACCGTGTGCTTATCGAAGACGACCGTTGGA
CAGGAGCATATGATGGTGGTAACGCGCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2G7HWR0

Antitoxin

Source ID Structure
AlphaFold DB A0A0N1EF65

References