Detailed information of TA system TA200405

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsaAT/-
• Location: 2245672..2246142
• Replicon: chromosome
• Accession: NZ_CP073855
• Organism: Staphylococcus epidermidis strain B1269488

Toxin (Protein)

• Gene name: TsaT
• Uniprot ID: Q5HQS5
• Locus tag: KFV48_RS10985
• Protein ID: WP_001829569.1
• Coordinates: 2245954..2246142 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: TsaA
• Uniprot ID: D4FNP2
• Locus tag: KFV48_RS13580
• Protein ID: WP_002438963.1
• Coordinates: 2245672..2245929 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFV48_RS10950 (KFV48_10970)	2241036..2241752	-	717	WP_001831974.1	type I 3-dehydroquinate dehydratase	-
KFV48_RS10955 (KFV48_10975)	2241913..2242335	+	423	WP_002457221.1	organic hydroperoxide resistance protein	-
KFV48_RS10960 (KFV48_10980)	2242519..2242674	-	156	WP_001829648.1	hypothetical protein	-
KFV48_RS10965 (KFV48_10985)	2243260..2243760	+	501	WP_001829623.1	GNAT family N-acetyltransferase	-
KFV48_RS10970 (KFV48_10990)	2244031..2244618	-	588	WP_001829615.1	histidine phosphatase family protein	-
KFV48_RS10975 (KFV48_10995)	2244832..2245068	+	237	WP_002457224.1	hypothetical protein	-
KFV48_RS10980 (KFV48_11000)	2245060..2245263	-	204	WP_002438966.1	sterile alpha motif-like domain-containing protein	-
KFV48_RS13580	2245672..2245929	+	258	WP_002438963.1	hypothetical protein	Antitoxin
KFV48_RS10985 (KFV48_11005)	2245954..2246142	+	189	WP_001829569.1	hypothetical protein	Toxin
KFV48_RS10990 (KFV48_11010)	2246332..2246886	+	555	WP_001829566.1	lipoprotein N-acylation protein LnsA	-
KFV48_RS10995 (KFV48_11015)	2246946..2247179	-	234	WP_001829664.1	hypothetical protein	-
KFV48_RS11000 (KFV48_11020)	2247463..2248209	+	747	WP_002438959.1	poly-gamma-glutamate hydrolase family protein	-
KFV48_RS11005 (KFV48_11025)	2248308..2248592	+	285	WP_001829635.1	hypothetical protein	-
KFV48_RS11010 (KFV48_11030)	2248616..2248840	+	225	WP_001829613.1	hypothetical protein	-
KFV48_RS11015 (KFV48_11035)	2249461..2249661	-	201	WP_001829681.1	cold-shock protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2236644..2260928	24284
	-	flank	IS/Tn	-	-	2242519..2242674	155

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7229.94 Da
• Isoelectric Point: 9.7844

>T200405 WP_001829569.1 NZ_CP073855:2245954-2246142 [Staphylococcus epidermidis]
MSLHFQILLWLSILFIIAGTILLVTMLKTKKEERKESYLGFTVIFLIFGFAILIYTFIFGIL

Nucleotide

• Length: 189 bp

>T200405 NZ_CP097841:113429-113734 [Shigella flexneri]
ATGCAGTTTAAGGTTTACGCCTATAAAAGAGAGAGTCGTTATCGTCTGTTTGTGGATGTACAGAGTGACATTATTGACAC
GCCCGGACGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGATAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACTGATATGGCCAGTGTGCCGATCTTCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 10014.05 Da
• Isoelectric Point: 4.5440

>AT200405 WP_002438963.1 NZ_CP073855:2245672-2245929 [Staphylococcus epidermidis]
MKLNKANIFNLIFTILFFSFNILITYNANIDYKLWLIPGLAICGFALFSSLTLVIIYSDLFSEILFFINIILALYYIYPI
FYEFV

Nucleotide

• Length: 258 bp

>AT200405 NZ_CP097841:113209-113427 [Shigella flexneri]
ATGAAGCAGCGTATTACTGTCACTATAGACAGTGACAGCTATCAGTTACTTAAATCCGCAAACGTGAATATCTCAGGCCT
GGTAAATACGGCCATGCAAAAAGAGGCCCGCCGTCTGCGTGCGGAACGCTGGCAGGCTGAAAATCAGCAGGGGATGGCTG
AGATCGCCCGTTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A0N0LUH8

Antitoxin

• AlphaFold DB: A0A0U5IVM3