Detailed information of TA system TA200396

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsaAT/-
• Location: 464827..465297
• Replicon: chromosome
• Accession: NZ_CP073852
• Organism: Staphylococcus epidermidis strain B1266915

Toxin (Protein)

• Gene name: TsaT
• Uniprot ID: Q5HQS5
• Locus tag: KFV26_RS02270
• Protein ID: WP_001829569.1
• Coordinates: 464827..465015 (-)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: TsaA
• Uniprot ID: D4FNP2
• Locus tag: KFV26_RS12905
• Protein ID: WP_002438963.1
• Coordinates: 465040..465297 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFV26_RS02240 (KFV26_02240)	461308..461508	+	201	WP_001829681.1	cold-shock protein	-
KFV26_RS02245 (KFV26_02245)	462129..462353	-	225	WP_001829613.1	hypothetical protein	-
KFV26_RS02250 (KFV26_02250)	462377..462661	-	285	WP_001829635.1	hypothetical protein	-
KFV26_RS02255 (KFV26_02255)	462760..463506	-	747	WP_002438959.1	poly-gamma-glutamate hydrolase family protein	-
KFV26_RS02260 (KFV26_02260)	463790..464023	+	234	WP_001829664.1	hypothetical protein	-
KFV26_RS02265 (KFV26_02265)	464083..464637	-	555	WP_001829566.1	lipoprotein N-acylation protein LnsA	-
KFV26_RS02270 (KFV26_02270)	464827..465015	-	189	WP_001829569.1	hypothetical protein	Toxin
KFV26_RS12905	465040..465297	-	258	WP_002438963.1	hypothetical protein	Antitoxin
KFV26_RS02275 (KFV26_02275)	465706..465909	+	204	WP_002438966.1	sterile alpha motif-like domain-containing protein	-
KFV26_RS02280 (KFV26_02280)	465901..466137	-	237	WP_002457224.1	hypothetical protein	-
KFV26_RS02285 (KFV26_02285)	466351..466938	+	588	WP_001829615.1	histidine phosphatase family protein	-
KFV26_RS02290 (KFV26_02290)	467209..467709	-	501	WP_001829623.1	GNAT family N-acetyltransferase	-
KFV26_RS02295 (KFV26_02295)	468313..468450	+	138	WP_162161215.1	hypothetical protein	-
KFV26_RS02300 (KFV26_02300)	468634..469056	-	423	WP_002457221.1	organic hydroperoxide resistance protein	-
KFV26_RS02305 (KFV26_02305)	469217..469933	+	717	WP_001831974.1	type I 3-dehydroquinate dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	446615..469933	23318
	-	flank	IS/Tn	-	-	468295..468450	155

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-83)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7229.94 Da
• Isoelectric Point: 9.7844

>T200396 WP_001829569.1 NZ_CP073852:c465015-464827 [Staphylococcus epidermidis]
MSLHFQILLWLSILFIIAGTILLVTMLKTKKEERKESYLGFTVIFLIFGFAILIYTFIFGIL

Nucleotide

• Length: 189 bp

>T200396 NZ_CP097840:c3212741-3212454 [Shigella flexneri]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 10014.05 Da
• Isoelectric Point: 4.5440

>AT200396 WP_002438963.1 NZ_CP073852:c465297-465040 [Staphylococcus epidermidis]
MKLNKANIFNLIFTILFFSFNILITYNANIDYKLWLIPGLAICGFALFSSLTLVIIYSDLFSEILFFINIILALYYIYPI
FYEFV

Nucleotide

• Length: 258 bp

>AT200396 NZ_CP097840:c3212980-3212741 [Shigella flexneri]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• AlphaFold DB: A0A0N0LUH8

Antitoxin

• AlphaFold DB: A0A0U5IVM3