Detailed information of TA system TA200391

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsaAT/-
• Location: 467385..467855
• Replicon: chromosome
• Accession: NZ_CP073850
• Organism: Staphylococcus epidermidis strain B1266911

Toxin (Protein)

• Gene name: TsaT
• Uniprot ID: Q5HQS5
• Locus tag: KFV33_RS02355
• Protein ID: WP_001829569.1
• Coordinates: 467385..467573 (-)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: TsaA
• Uniprot ID: D4FNP2
• Locus tag: KFV33_RS13320
• Protein ID: WP_002438963.1
• Coordinates: 467598..467855 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFV33_RS02325 (KFV33_02325)	463866..464066	+	201	WP_001829681.1	cold-shock protein	-
KFV33_RS02330 (KFV33_02330)	464687..464911	-	225	WP_001829613.1	hypothetical protein	-
KFV33_RS02335 (KFV33_02335)	464935..465219	-	285	WP_001829635.1	hypothetical protein	-
KFV33_RS02340 (KFV33_02340)	465318..466064	-	747	WP_002438959.1	poly-gamma-glutamate hydrolase family protein	-
KFV33_RS02345 (KFV33_02345)	466348..466581	+	234	WP_001829664.1	hypothetical protein	-
KFV33_RS02350 (KFV33_02350)	466641..467195	-	555	WP_001829566.1	lipoprotein N-acylation protein LnsA	-
KFV33_RS02355 (KFV33_02355)	467385..467573	-	189	WP_001829569.1	hypothetical protein	Toxin
KFV33_RS13320	467598..467855	-	258	WP_002438963.1	hypothetical protein	Antitoxin
KFV33_RS02360 (KFV33_02360)	468264..468467	+	204	WP_002438966.1	sterile alpha motif-like domain-containing protein	-
KFV33_RS02365 (KFV33_02365)	468459..468695	-	237	WP_002457224.1	hypothetical protein	-
KFV33_RS02370 (KFV33_02370)	468909..469496	+	588	WP_001829615.1	histidine phosphatase family protein	-
KFV33_RS02375 (KFV33_02375)	469767..470267	-	501	WP_001829623.1	GNAT family N-acetyltransferase	-
KFV33_RS02380 (KFV33_02380)	470853..471008	+	156	WP_001829648.1	hypothetical protein	-
KFV33_RS02385 (KFV33_02385)	471192..471614	-	423	WP_002457221.1	organic hydroperoxide resistance protein	-
KFV33_RS02390 (KFV33_02390)	471775..472491	+	717	WP_001831974.1	type I 3-dehydroquinate dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	470853..471008	155

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (30-81)

Antitoxin

• Uncharacterised protein family HicB (60-112)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7229.94 Da
• Isoelectric Point: 9.7844

>T200391 WP_001829569.1 NZ_CP073850:c467573-467385 [Staphylococcus epidermidis]
MSLHFQILLWLSILFIIAGTILLVTMLKTKKEERKESYLGFTVIFLIFGFAILIYTFIFGIL

Nucleotide

• Length: 189 bp

>T200391 NZ_CP097840:1235434-1235709 [Shigella flexneri]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGAAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
TGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 10014.05 Da
• Isoelectric Point: 4.5440

>AT200391 WP_002438963.1 NZ_CP073850:c467855-467598 [Staphylococcus epidermidis]
MKLNKANIFNLIFTILFFSFNILITYNANIDYKLWLIPGLAICGFALFSSLTLVIIYSDLFSEILFFINIILALYYIYPI
FYEFV

Nucleotide

• Length: 258 bp

>AT200391 NZ_CP097840:1235706-1236065 [Shigella flexneri]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCAGCTGTTATTACCTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGTATTCAGGGGCTGCAAAAAGAAGGAG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Structures

Toxin

• AlphaFold DB: A0A0N0LUH8

Antitoxin

• AlphaFold DB: A0A0U5IVM3