Detailed information of TA system TA020039

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/-
• Location: 2894849..2895432
• Replicon: chromosome
• Accession: CP009789
• Organism: Escherichia coli K-12 strain ER3413

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: ER3413_2867
• Protein ID: AIZ52583.1
• Coordinates: 2894849..2895184 (-)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: ER3413_2868
• Protein ID: AIZ52584.1
• Coordinates: 2895184..2895432 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ER3413_2864	2890736..2892034	-	1299	AIZ52580.1	enolase	-
ER3413_2865	2892122..2893759	-	1638	AIZ52581.1	CTP synthetase	-
ER3413_2866	2893987..2894778	-	792	AIZ52582.1	nucleoside triphosphate pyrophosphohydrolase	-
ER3413_2867	2894849..2895184	-	336	AIZ52583.1	mRNA interferase toxin, antitoxin is MazE	Toxin
ER3413_2868	2895184..2895432	-	249	AIZ52584.1	antitoxin of the ChpA-ChpR toxin-antitoxin system	Antitoxin
ER3413_2869	2895510..2897744	-	2235	AIZ52585.1	(p)ppGpp synthetase I/GTP pyrophosphokinase	-
ER3413_2870	2897792..2899093	-	1302	AIZ52587.1	23S rRNA m(5)U1939 methyltransferase, SAM-dependent	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-109)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12098.04 Da
• Isoelectric Point: 8.2618

>T20039 AIZ52583.1 CP009789:c2895184-2894849 [Escherichia coli K-12]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Nucleotide

• Length: 336 bp

>T20039 CP009789:c2895184-2894849 [Escherichia coli K-12]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9355.74 Da
• Isoelectric Point: 4.4665

>AT20039 AIZ52584.1 CP009789:c2895432-2895184 [Escherichia coli K-12]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Nucleotide

• Length: 249 bp

>AT20039 CP009789:c2895432-2895184 [Escherichia coli K-12]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA