Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2028170..2028699 Replicon chromosome
Accession NZ_CP073847
Organism Staphylococcus epidermidis strain B1266875

Toxin (Protein)


Gene name mazF Uniprot ID Q5HME7
Locus tag KFV50_RS09720 Protein ID WP_001829891.1
Coordinates 2028170..2028532 (-) Length 121 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID Q5HME6
Locus tag KFV50_RS09725 Protein ID WP_001829931.1
Coordinates 2028529..2028699 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFV50_RS09700 (KFV50_09710) 2025171..2025941 - 771 WP_002468952.1 RNA polymerase sigma factor SigB -
KFV50_RS09705 (KFV50_09715) 2025955..2026395 - 441 WP_063278490.1 anti-sigma B factor RsbW -
KFV50_RS09710 (KFV50_09720) 2026397..2026723 - 327 WP_001829952.1 anti-sigma factor antagonist -
KFV50_RS09715 (KFV50_09725) 2026823..2027824 - 1002 WP_001829902.1 PP2C family protein-serine/threonine phosphatase -
KFV50_RS09720 (KFV50_09730) 2028170..2028532 - 363 WP_001829891.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
KFV50_RS09725 (KFV50_09735) 2028529..2028699 - 171 WP_001829931.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
KFV50_RS09730 (KFV50_09740) 2028786..2029934 - 1149 WP_002468951.1 alanine racemase -
KFV50_RS09735 (KFV50_09745) 2030000..2030353 - 354 WP_001829915.1 holo-ACP synthase -
KFV50_RS09740 (KFV50_09750) 2030401..2030910 - 510 WP_001829888.1 PH domain-containing protein -
KFV50_RS09745 (KFV50_09755) 2030897..2032402 - 1506 WP_001829976.1 PH domain-containing protein -
KFV50_RS09750 (KFV50_09760) 2032395..2032874 - 480 WP_063278489.1 PH domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13497.65 Da        Isoelectric Point: 9.9522

>T200388 WP_001829891.1 NZ_CP073847:c2028532-2028170 [Staphylococcus epidermidis]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTFLSESKMIEVDNALDISLGLNNFDHHKS

Download         Length: 363 bp

>T200388 NZ_CP097840:1154696-1154803 [Shigella flexneri]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAG

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6289.92 Da        Isoelectric Point: 4.1920

>AT200388 WP_001829931.1 NZ_CP073847:c2028699-2028529 [Staphylococcus epidermidis]
MLSFNQNRNHSLEQSLKEGYAQMADLNLSLATEAFPIECEACDCNESHLISNSKNE

Download         Length: 171 bp

>AT200388 NZ_CP097840:c1154647-1154581 [Shigella flexneri]
GTCTAGGTTCAAGATTAGCCCCCGTTATGTTGTCAGGTACATACCTGCAACGTGCGGGGGTTTTCTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2G7HWR0

Antitoxin

Source ID Structure
AlphaFold DB A0A0N1EF65

References