Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 693000..693529 Replicon chromosome
Accession NZ_CP073841
Organism Staphylococcus epidermidis strain B1264787

Toxin (Protein)


Gene name mazF Uniprot ID Q5HME7
Locus tag KFV40_RS03515 Protein ID WP_001829891.1
Coordinates 693167..693529 (+) Length 121 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID Q5HME6
Locus tag KFV40_RS03510 Protein ID WP_001829931.1
Coordinates 693000..693170 (+) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFV40_RS03485 (KFV40_03485) 688825..689304 + 480 WP_063278489.1 PH domain-containing protein -
KFV40_RS03490 (KFV40_03490) 689297..690802 + 1506 WP_001829976.1 PH domain-containing protein -
KFV40_RS03495 (KFV40_03495) 690789..691298 + 510 WP_001829888.1 PH domain-containing protein -
KFV40_RS03500 (KFV40_03500) 691346..691699 + 354 WP_001829915.1 holo-ACP synthase -
KFV40_RS03505 (KFV40_03505) 691765..692913 + 1149 WP_002468951.1 alanine racemase -
KFV40_RS03510 (KFV40_03510) 693000..693170 + 171 WP_001829931.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
KFV40_RS03515 (KFV40_03515) 693167..693529 + 363 WP_001829891.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
KFV40_RS03520 (KFV40_03520) 693875..694876 + 1002 WP_001829902.1 PP2C family protein-serine/threonine phosphatase -
KFV40_RS03525 (KFV40_03525) 694976..695302 + 327 WP_001829952.1 anti-sigma factor antagonist -
KFV40_RS03530 (KFV40_03530) 695304..695744 + 441 WP_063278490.1 anti-sigma B factor RsbW -
KFV40_RS03535 (KFV40_03535) 695758..696528 + 771 WP_002468952.1 RNA polymerase sigma factor SigB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13497.65 Da        Isoelectric Point: 9.9522

>T200378 WP_001829891.1 NZ_CP073841:693167-693529 [Staphylococcus epidermidis]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTFLSESKMIEVDNALDISLGLNNFDHHKS

Download         Length: 363 bp

>T200378 NZ_CP097837:c3697680-3697366 [Shigella flexneri]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6289.92 Da        Isoelectric Point: 4.1920

>AT200378 WP_001829931.1 NZ_CP073841:693000-693170 [Staphylococcus epidermidis]
MLSFNQNRNHSLEQSLKEGYAQMADLNLSLATEAFPIECEACDCNESHLISNSKNE

Download         Length: 171 bp

>AT200378 NZ_CP097837:c3697369-3696953 [Shigella flexneri]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2G7HWR0

Antitoxin

Source ID Structure
AlphaFold DB A0A0N1EF65

References