Detailed information of TA system TA200371

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsaAT/-
• Location: 466263..466733
• Replicon: chromosome
• Accession: NZ_CP073840
• Organism: Staphylococcus epidermidis strain B1264454

Toxin (Protein)

• Gene name: TsaT
• Uniprot ID: Q5HQS5
• Locus tag: KFV39_RS02350
• Protein ID: WP_001829569.1
• Coordinates: 466263..466451 (-)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: TsaA
• Uniprot ID: D4FNP2
• Locus tag: KFV39_RS13280
• Protein ID: WP_002438963.1
• Coordinates: 466476..466733 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFV39_RS02320 (KFV39_02320)	462744..462944	+	201	WP_001829681.1	cold-shock protein	-
KFV39_RS02325 (KFV39_02325)	463565..463789	-	225	WP_001829613.1	hypothetical protein	-
KFV39_RS02330 (KFV39_02330)	463813..464097	-	285	WP_001829635.1	hypothetical protein	-
KFV39_RS02335 (KFV39_02335)	464196..464942	-	747	WP_002438959.1	poly-gamma-glutamate hydrolase family protein	-
KFV39_RS02340 (KFV39_02340)	465226..465459	+	234	WP_001829664.1	hypothetical protein	-
KFV39_RS02345 (KFV39_02345)	465519..466073	-	555	WP_001829566.1	lipoprotein N-acylation protein LnsA	-
KFV39_RS02350 (KFV39_02350)	466263..466451	-	189	WP_001829569.1	hypothetical protein	Toxin
KFV39_RS13280	466476..466733	-	258	WP_002438963.1	hypothetical protein	Antitoxin
KFV39_RS02355 (KFV39_02355)	467142..467345	+	204	WP_002438966.1	sterile alpha motif-like domain-containing protein	-
KFV39_RS02360 (KFV39_02360)	467337..467573	-	237	WP_002457224.1	hypothetical protein	-
KFV39_RS02365 (KFV39_02365)	467787..468374	+	588	WP_001829615.1	histidine phosphatase family protein	-
KFV39_RS02370 (KFV39_02370)	468645..469145	-	501	WP_001829623.1	GNAT family N-acetyltransferase	-
KFV39_RS02375 (KFV39_02375)	469731..469886	+	156	WP_001829648.1	hypothetical protein	-
KFV39_RS02380 (KFV39_02380)	470070..470492	-	423	WP_002457221.1	organic hydroperoxide resistance protein	-
KFV39_RS02385 (KFV39_02385)	470653..471369	+	717	WP_001831974.1	type I 3-dehydroquinate dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	451477..469886	18409
	-	flank	IS/Tn	-	-	469731..469886	155

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (5-70)

Antitoxin

• Cro/C1-type helix-turn-helix domain (44-97)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7229.94 Da
• Isoelectric Point: 9.7844

>T200371 WP_001829569.1 NZ_CP073840:c466451-466263 [Staphylococcus epidermidis]
MSLHFQILLWLSILFIIAGTILLVTMLKTKKEERKESYLGFTVIFLIFGFAILIYTFIFGIL

Nucleotide

• Length: 189 bp

>T200371 NZ_CP097837:c1239913-1239683 [Shigella flexneri]
ATGAAGGTATACGGCAGCAATCTCGGAGAACCTCACACCAGCGCCTTTGGTGACGGGCTTTTCGAACTCAGAATTAAAGG
CAACGATGGGATCGCGCGTGTTTTTTACTGCACTCTGACAGGGAAACGCATCATCATGCTGCATAGTTTTGTAAAGAAAG
CGCAGAAAACACCGCCAGCCGAACGCAAGAAAGCTGAAACCAGAATGAAGGAGGTTAAGCATGACTGGTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 10014.05 Da
• Isoelectric Point: 4.5440

>AT200371 WP_002438963.1 NZ_CP073840:c466733-466476 [Staphylococcus epidermidis]
MKLNKANIFNLIFTILFFSFNILITYNANIDYKLWLIPGLAICGFALFSSLTLVIIYSDLFSEILFFINIILALYYIYPI
FYEFV

Nucleotide

• Length: 258 bp

>AT200371 NZ_CP097837:c1239693-1239385 [Shigella flexneri]
ATGACTGGTAAACGCACTCTCCCCACCATGACACACGACGAAATGGCAGCCAAATGGATGGAAGACCCGGCTTTTAAAGC
AGAATACGACGCTATCGCTGACGAATTCGCACTGCTTGATGAAATGCTGGCAGCACGCAAAGAAGCTGGCTTAACTCAGG
CTGAAGTTGCCGAGCGAATGGGAACAAAAGCGACCGCGATCACCAGAATGGAAAGTAATCTCGCATCAGGTATCAGCGGC
CCATCATTTGCCACACTAAAAAAATTCGCCCGCGCTACTGGAAAAAAACTCCAGATCCGCTTCGTTTAA

Structures

Toxin

• AlphaFold DB: A0A0N0LUH8

Antitoxin

• AlphaFold DB: A0A0U5IVM3