Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2059500..2060029 Replicon chromosome
Accession NZ_CP073835
Organism Staphylococcus epidermidis strain B1230143

Toxin (Protein)


Gene name mazF Uniprot ID Q5HME7
Locus tag KFV52_RS10170 Protein ID WP_001829891.1
Coordinates 2059500..2059862 (-) Length 121 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID Q5HME6
Locus tag KFV52_RS10175 Protein ID WP_001829931.1
Coordinates 2059859..2060029 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFV52_RS10150 (KFV52_10160) 2056501..2057271 - 771 WP_002468952.1 RNA polymerase sigma factor SigB -
KFV52_RS10155 (KFV52_10165) 2057246..2057725 - 480 WP_001829903.1 anti-sigma B factor RsbW -
KFV52_RS10160 (KFV52_10170) 2057727..2058053 - 327 WP_001829952.1 anti-sigma factor antagonist -
KFV52_RS10165 (KFV52_10175) 2058153..2059154 - 1002 WP_001829902.1 PP2C family protein-serine/threonine phosphatase -
KFV52_RS10170 (KFV52_10180) 2059500..2059862 - 363 WP_001829891.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
KFV52_RS10175 (KFV52_10185) 2059859..2060029 - 171 WP_001829931.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
KFV52_RS10180 (KFV52_10190) 2060116..2061264 - 1149 WP_002468951.1 alanine racemase -
KFV52_RS10185 (KFV52_10195) 2061330..2061683 - 354 WP_001829915.1 holo-ACP synthase -
KFV52_RS10190 (KFV52_10200) 2061731..2062240 - 510 WP_001829888.1 PH domain-containing protein -
KFV52_RS10195 (KFV52_10205) 2062227..2063732 - 1506 WP_001829976.1 PH domain-containing protein -
KFV52_RS10200 (KFV52_10210) 2063725..2064204 - 480 WP_001829909.1 PH domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13497.65 Da        Isoelectric Point: 9.9522

>T200363 WP_001829891.1 NZ_CP073835:c2059862-2059500 [Staphylococcus epidermidis]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTFLSESKMIEVDNALDISLGLNNFDHHKS

Download         Length: 363 bp

>T200363 NZ_CP097836:c13369-13064 [Shigella flexneri]
ATGCAGTTTAAGGTTTACGCCTATAAAAGAGAGAGTCGTTATCGTCTGTTTGTGGATGTACAGAGTGACATTATTGACAC
GCCCGGACGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGATAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACTGATATGGCCAGTGTGCCGATCTTCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6289.92 Da        Isoelectric Point: 4.1920

>AT200363 WP_001829931.1 NZ_CP073835:c2060029-2059859 [Staphylococcus epidermidis]
MLSFNQNRNHSLEQSLKEGYAQMADLNLSLATEAFPIECEACDCNESHLISNSKNE

Download         Length: 171 bp

>AT200363 NZ_CP097836:c13589-13371 [Shigella flexneri]
ATGAAGCAGCGTATTACTGTCACTATAGACAGTGACAGCTATCAGTTACTTAAATCCGCAAACGTGAATATCTCAGGCCT
GGTAAATACGGCCATGCAAAAAGAGGCCCGCCGTCTGCGTGCGGAACGCTGGCAGGCTGAAAATCAGCAGGGGATGGCTG
AGATCGCCCGTTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2G7HWR0

Antitoxin

Source ID Structure
AlphaFold DB A0A0N1EF65

References