Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2022196..2022725 Replicon chromosome
Accession NZ_CP073830
Organism Staphylococcus epidermidis strain B1220165

Toxin (Protein)


Gene name mazF Uniprot ID Q5HME7
Locus tag KFV27_RS09680 Protein ID WP_001829891.1
Coordinates 2022196..2022558 (-) Length 121 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID Q5HME6
Locus tag KFV27_RS09685 Protein ID WP_001829931.1
Coordinates 2022555..2022725 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFV27_RS09660 (KFV27_09670) 2019197..2019967 - 771 WP_002468952.1 RNA polymerase sigma factor SigB -
KFV27_RS09665 (KFV27_09675) 2019981..2020421 - 441 WP_063278490.1 anti-sigma B factor RsbW -
KFV27_RS09670 (KFV27_09680) 2020423..2020749 - 327 WP_001829952.1 anti-sigma factor antagonist -
KFV27_RS09675 (KFV27_09685) 2020849..2021850 - 1002 WP_001829902.1 PP2C family protein-serine/threonine phosphatase -
KFV27_RS09680 (KFV27_09690) 2022196..2022558 - 363 WP_001829891.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
KFV27_RS09685 (KFV27_09695) 2022555..2022725 - 171 WP_001829931.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
KFV27_RS09690 (KFV27_09700) 2022812..2023960 - 1149 WP_002468951.1 alanine racemase -
KFV27_RS09695 (KFV27_09705) 2024026..2024379 - 354 WP_001829915.1 holo-ACP synthase -
KFV27_RS09700 (KFV27_09710) 2024427..2024936 - 510 WP_001829888.1 PH domain-containing protein -
KFV27_RS09705 (KFV27_09715) 2024923..2026428 - 1506 WP_001829976.1 PH domain-containing protein -
KFV27_RS09710 (KFV27_09720) 2026421..2026900 - 480 WP_063278489.1 PH domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13497.65 Da        Isoelectric Point: 9.9522

>T200358 WP_001829891.1 NZ_CP073830:c2022558-2022196 [Staphylococcus epidermidis]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTFLSESKMIEVDNALDISLGLNNFDHHKS

Download         Length: 363 bp

>T200358 NZ_CP097834:3628939-3629046 [Shigella flexneri]
ATGACGCTCGCAGAACTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6289.92 Da        Isoelectric Point: 4.1920

>AT200358 WP_001829931.1 NZ_CP073830:c2022725-2022555 [Staphylococcus epidermidis]
MLSFNQNRNHSLEQSLKEGYAQMADLNLSLATEAFPIECEACDCNESHLISNSKNE

Download         Length: 171 bp

>AT200358 NZ_CP097834:c3628882-3628827 [Shigella flexneri]
TCAAGAATGGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2G7HWR0

Antitoxin

Source ID Structure
AlphaFold DB A0A0N1EF65

References